SnapShot: Nonmotor Proteins in Spindle Assembly

*DDA3 Human, mouse Spindle microtubules, midbody Bundles microtubules; regulates the spindle pole localized microtubule depolymerase Kif2a Fidgetin Human, mouse (Fignl1), fl y (Fignl1), worm (FIGL-1), frog (Fignl1) Centrosomes Catalyzes turnover of γ-tubulin; contributes to microtubule depolymeriza-tion and chromosome movement Growing microtubule plus ends Stabilizes microtubule plus ends; infl uences chromosome alignment CLASP1, 2 Human, mouse, fl y (MAST/Orbit), worm (CLS-2) Sp (Peg1) Growing microtubule plus ends Stabilizes plus-end microtubule dynamics; infl uences spindle formation and chromosome alignment Growing microtubule plus ends Regulates growth of microtubule plus ends by suppressing depolymerization; infl uences chromosome alignment chromosomal Passenger complex *Aurora B Human, mouse (AIM-1), fl y (Lal), worm (AIR-2) frog, Sc (Ipl1), Sp (ARK1) Inner centromeres in metaphase, central spindle/midbody in anaphase Inner centromeres in metaphase, central spindle/midbody in anaphase Controls the localization and activity of Aurora B Inner centromeres in metaphase, central spindle/midbody in anaphase Controls the localization and activity of Aurora B Inner centromeres in metaphase, central spindle/midbody in anaphase Controls the localization and activity of Aurora B; protects against apoptosis TD60 Human, mouse, fl y, frog Inner centromeres in metaphase, central spindle/midbody in anaphase Activates the kinase activity of Aurora B others CHICA Human Spindle microtubules Polar ejection in cooperation with the chromokinesin Kid Chromator Fly Fusiform spindle shape around spindle microtubules Forms a microtubule-independent fusiform spindle; infl uences bipolar spindle formation; putative spindle matrix protein Coactivator of dynein, required for dynein functions including spindle pole focusing and chromosome movement *Lamin B Human, mouse, fl y, worm, frog Spindle microtubules Promotes microtubule assembly and organization; putative spindle matrix protein Megator Fly Fusiform spindle shape around spindle microtubules Forms complex with Skeletor/Chromator; forms microtubule-independent fusiform spindle; putative spindle matrix protein Skeletor Fly Fusiform spindle shape around spindle microtubules Forms a microtubule-independent fusiform spindle; putative spindle matrix protein *Tankyrase Human, mouse, fl y, worm (PME-5), frog Spindle poles Contributes to spindle pole focusing; putative spindle matrix protein Vik1 Sc Spindle pole body Infl uences microtubule stability by regulation of the kinesin Kar3