Automated assay of hexosaminidases in serum.
نویسندگان
چکیده
In serum there are two classes of hexosaminidase. One of these is heat-labile and is inactive in children with Tay-Sachs disease. Serum from heterozygotes for Tay-Sachs disease has half the normal activity of the heat-labile enzyme. The heat-labile enzyme can be inactivated by heating serum diluted in buffer for 5 mm at 60 #{176}C. We have used this property to develop a reliable, fully automated assay for serum hexosaminidases, which can be used for mass carrier screening programs.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 19 12 شماره
صفحات -
تاریخ انتشار 1973