A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinase.
نویسندگان
چکیده
A novel label-free electrochemical strategy for monitoring the activity and inhibition of protein kinase is developed, based on the linkage between the phosphorylated peptide and DNA functionalized Au nanoparticles (DNA-AuNPs) by Zr(4+) and the chronocoulometric response of [Ru(NH(3))(6)](3+) absorbed on the DNA-AuNPs.
منابع مشابه
A DNA-based electrochemical strategy for label-free monitoring the activity and inhibition of protein kinasew
Protein phosphorylation by kinase plays a significant regulatory role in many vital biological processes involving metabolic pathways, cellular signal communications, neural activities, and so on. Aberrant protein phosphorylation states and kinase activity are associated with many human diseases. The identification of kinase activities and their potential inhibitors is not only necessary for un...
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ورودعنوان ژورنال:
- Chemical communications
دوره 45 شماره
صفحات -
تاریخ انتشار 2009