optimization of culture conditions and medium components for carboxymethyl cellulase (cmcase) production by a novel basidiomycete strain peniophora sp. ndvn01

background: cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. objectives: in this study, we described the identification of a basidiomycete isolate ndvn01 and optimization of culture conditions and medium components for cmcase production by this strain under liquid state fermentation. the cmcase was estimated as 32 - 33 kda on a native polyacrylamide gel electrophoresis (page). materials and methods: we used 5 basidiomycetes for screening cmcase production, internal transcribed spacer (its) sequence analysis in combination with morphology for strain identification, and liquid state fermentation for optimization of cmcase production. results: the maximum cmcase production by peniophora sp. ndvn01 was obtained at 28°c, with the initial medium ph of 7 and within 120 hours of cultivation in the optimum medium containing 80% (v/v) of potato infusion, 0.6% (w/v) straw rice as additional carbon source and 0.2% (w/v) ammonium hydrogen phosphate as an additional nitrogen source, and 0.5% (w/v) pulp as inducer. conclusions: under optimal conditions, peniophora sp. ndvn01 produced 24.65 ± 0.37 units of cmcase per ml of culture supernatant, which was 8.6 times higher than the amount (2.87 ± 0.28 u.ml -1) before optimization.