production of recombinant human granulocyte-colony stimulating factor by pichia pastoris
نویسندگان
چکیده
human granulocyte-colony stimulating factor (hg-csf) cdna was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase (aox1) promoter. an expression vector for hg-csf secretion was constructed using vector ppic9. higher levels of hg-csf was obtained using a p. pastoris mut+ (methanol utilization fast) phenotype. the effects of environmental factors such as temperature and ph on the p. pastoris cell growth and hg-csf production during fermentation were investigated. cell growth and hg-csf production were found to be optimal at 28°c and ph 6.0. a fed-batch fermentation process was also developed to obtain high cell density and higher levels of protein expression. using a high cell density cultivation method, cell dry weight and hg-csf concentration reached 100 g/l and 35 mg/l, respectively.
منابع مشابه
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...
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عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 5
شماره 3 2007
کلمات کلیدی
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