effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (bubalus bubalis)
نویسندگان
چکیده
the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and viability were evaluated at 0 (t0) (immediately after dilution), 60 (t1) and 120 (t2) min after diluting semen. in the second step, semen samples were diluted with tris-egg yolk-glycerol extender containing the same amounts of sodium selenite, cooled to 4 ˚c, equilibrated and semen parameters (motility, viability, membrane integrity and dna damage) were estimated. then, the semen was packed in 0.5 ml french straws and frozen in liquid nitrogen. later, the semen was thawed and analyzed for the same parameters, as well as total antioxidant capacity. results showed that addition of 1 and 2 μgml-1 selenium to the semen extender significantly increased the sperm motility of fresh and equilibrated semen compared to the control without affecting other parameters. however, in frozen-thawed semen, extenders containing 1 and 2 μg ml-1 selenium significantly improved sperm motility, viability, membrane integrity and semen total antioxidant capacity and also resulted in lower dna damaged sperms. in this study selenium supplementation of semen extender of 4 and 8 μg ml-1 had deleterious effects on sperm parameters as early as the samples were prepared for freezing.
منابع مشابه
Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis)
The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...
متن کاملEffects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis)
The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg mL(-1) sodium selenite and the sperm motility a...
متن کاملEffects of In Vitro Zinc Sulphate Additive to The Semen Extender on Water Buffalo (Bubalusbubalis) Spermatozoa before and after Freezing
متن کامل
P-142: Effects of A Synthetic Antioxidant (4-Hydroxy Tempo) Additive to The Semen Extender on the Ejaculated Spermatozoa Characteristics before and after Freezing in Water Buffaloes (Bubalus Bubalis)
Background: Semen cryopreservation is the most important section of artificial insemination programs; it allows preservation of semen fertility for a long time. Materials and Methods: The aim of the present study was to detect the effect of in vitro supplementation of 4-hydroxy Tempo on fresh and frozen spermatozoa quality of buffalo bulls. Five healthy buffalo bulls (5 ejaculates from each bul...
متن کاملeffects of in vitro zinc sulphate additive to the semen extender on water buffalo (bubalusbubalis) spermatozoa before and after freezing
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متن کاملEffects of In Vitro Zinc Sulphate Additive to The Semen Extender on Water Buffalo (Bubalusbubalis) Spermatozoa before and after Freezing
BACKGROUND The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. MATERIALS AND METHODS In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occa...
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عنوان ژورنال:
veterinary research forumناشر: faculty of veterinary medicine, urmia university
ISSN 2008-8140
دوره 3
شماره 4 2012
کلمات کلیدی
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