evaluation of immunization of balb/c mice by plasmid encoding complete rop2 gene of toxoplasma gondii

objective: toxoplasma gondii is an obligate intracellular protozoan that causes toxoplasmosis in human and animal. in recent years, significant progress has been made in the identification of vaccine candidates which can induce protective responses. in this study we used complete rhoptry protein 2 gene of toxoplasma gondii as a single dna vaccine and evaluated its immune responses in comparison with control groups. materials and methods: balb/c mice were immunized intramuscularly with three weaks time interval with pcrop2 (as case group) and pc-dna3 and pbs (as control groups). after immunization, we evaluated the immune response using cytokine and antibody measurements. results: the results of cytokine (ifn-γ, il-4) assays showed that mice immunized with pcrop2, elicited stronger th1-type cellular immune responses than those immunized with empty plasmid, or pbs (high level of ifn-γ and low-level of il-4).also anti-t. gondii igg titres (od) increased markedly in the pcrop2 group, which was significantly higher than those of control groups (p