evaluation of cr (vi) induced neurotoxicity and oxidative stress in pc12 cells

objective: hexavalent chromium [cr (vi)] compounds are well-known environmental contaminants  generated from industrial processes. several studies have reported the harmful effects of cr (vi) on different organs, however, little is known about neurotoxic effects of cr (vi). the aim of this study is to investigate the toxic effects of cr (vi) on pc12 cells. methods: pc12 cells were cultured following standard protocol and exposed to various concentrations (1-100 μm) of potassium dichromate (k2cr2o7) for 24, 48 and 72 h. after exposure, cell viability was measured by the mtt assy. after exposure, production of reactive oxygen species (ros) and lipid peroxidation were measured. results: potassium dichromate induced significant cell death in pc12 cells. the ic50 values for cytotoxicity were 22.02 for 24 h, 1.88 for 48 h, and 1.85 for 72 h of exposure. significant differences between ic50 for 24 h of exposure compared to 8 and 72 h of exposure were observed (p