genetic variation in the narrow-clawed crayfish (astacus leptodactylus) populations as assessed by pcr-rflp of mitochondrial coi gene
نویسندگان
چکیده
the genetic variation and population structure of narrow-clawed crayfish (astacus leptodactylus) was examined by means of polymerase chain reaction (pcr) restriction fragment length polymorphism (rflp) analysis of the cytochrome oxidase subunit i (coi) of mitochondrial dna. a total of 194 adult specimens were collected from seven sample sites including, two in the south caspian sea and one each in anzali wetland and aras reservoir and three rivers chafrood, masule rudkhan and siah darvishan. the pcr products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (ddeі, mboі, taqi, rsaі and hinfі). twenty eight composite haplotypes were showed with the number of haplotypes in each population sample ranging from 8 to 13. private haplotypes were found at very low frequencies. two regional (siah darvishan river and astara) groups were clearly recognized by cluster and molecular variance model (amova) analyses (p
منابع مشابه
Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene
The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each...
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Crayfish stocks collapsed in Turkey in mid 1980’s due to crayfish plague epidemics, with up to 25% survival in some cases. The collapsed stocks have then recovered slowly and commercial crayfish fishery was reinitiated gradually within a decade. We examined the prevalence of Aphanomyces astaciDNA in the narrow clawed crayfish (Astacus leptodactylus) collected from two Turkish lakes, Lake İznik ...
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Dietary cholesterol has been shown to be essential for several crustacean species. However, cholesterol requirement of narrow-clawed crayfish has not been reported. Therefore, a 12-week feeding trial was conducted to determine deitary cholesterol requirement of narrow-clawed crayfish. Juvenile crayfish with an average body weight of 13.8±1 g mean weight were intensively stocked in an indoor rec...
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2,4-D is a widely used phenoxy herbicide, potentially toxic to humans and biota. The objective of the present study was to reveal short term sublethal effects of 2,4-D on narrow-clawed freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823), based on histology, total haemocyte counts, selected haemolymph parameters, and oxidative stress parameters. In the laboratory conditions crayfish sp...
متن کاملEvaluation of different dietary protein and energy levels on growth performance and body composition of narrow clawed crayfish (Astacus leptodactylus)
متن کامل
Growth, Molting and Survival Response of Juvenile Narrow Clawed Crayfish, Astacus leptodactylus, Fed Two Sources of Dietary Oils
The influence of five pure diets of isonitrogenous and isoenergic with different ratios of Clupeidae fish oil (F) and soybean oil (S) on growth, molting and survival of narrow-clawed crayfish, Astacus leptodactylus, was evaluated during an 8-weeks trial. As soybean oil replaced with fish oil in each treatment, gently. Test diets were inclusive F0/S100, F30/S70, F50/S50, F70/S30 and F100/S0 fis...
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عنوان ژورنال:
molecular biology research communicationsناشر: shiraz university press
ISSN 2322-181X
دوره 4
شماره 4 2015
کلمات کلیدی
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