fully automated cdna microarray segmentation using a novel fuzzy-based algorithm
نویسندگان
چکیده
dna microarray is a powerful approach to study simultaneously, the expression of 1000 of genes in a single experiment. the average value of the fluorescent intensity could be calculated in a microarray experiment. the calculated intensity values are very close in amount to the levels of expression of a particular gene. however, determining the appropriate position of every spot in microarray images is a main challenge, which leads to the accurate classification of normal and abnormal (cancer) cells. in this paper, first a preprocessing approach is performed to eliminate the noise and artifacts available in microarray cells using the nonlinear anisotropic diffusion filtering method. then, the coordinate center of each spot is positioned utilizing the mathematical morphology operations. finally, the position of each spot is exactly determined through applying a novel hybrid model based on the principle component analysis and the spatial fuzzy c‑means clustering (sfcm) algorithm. using a gaussian kernel in sfcm algorithm will lead to improving the quality in complementary dna microarray segmentation. the performance of the proposed algorithm has been evaluated on the real microarray images, which is available in stanford microarray databases. results illustrate that the accuracy of microarray cells segmentation in the proposed algorithm reaches to 100% and 98% for noiseless/noisy cells, respectively.
منابع مشابه
cDNA microarray image segmentation using root signals
A vector processing based framework suitable for cDNA microarray image segmentation is introduced and analyzed in this paper. By using nonlinear, generalized selection vector filters the framework proposed here classifies the cDNA image data as either microarray spots or image background. The solution converges to a root signal that represents the segmented cDNA microarray image with the regula...
متن کاملSpotted cDNA microarray image segmentation using ACWE
The segmentation of the picture (and, implicitly, the segmentation method used) is one of the three important steps in microarray image processing, together with spot gridding and information extraction. It directly affects the accuracy of gene expression analysis in the data mining process that follows. In 2001 a segmentation method for arbitrary pictures using the Active Contours Without Edge...
متن کاملCrossword: A Fully Automated Algorithm for the Segmentation and Quality Control of Protein Microarray Images
Biological assays formatted as microarrays have become a critical tool for the generation of the comprehensive data sets required for systems-level understanding of biological processes. Manual annotation of data extracted from images of microarrays, however, remains a significant bottleneck, particularly for protein microarrays due to the sensitivity of this technology to weak artifact signal....
متن کاملA novel neural network approach to cDNA microarray image segmentation
Microarray technology has become a great source of information for biologists to understand the workings of DNA which is one of the most complex codes in nature. Microarray images typically contain several thousands of small spots, each of which represents a different gene in the experiment. One of the key steps in extracting information from a microarray image is the segmentation whose aim is ...
متن کاملA Novel Pixon-Based Image Segmentation Process Using Fuzzy Filtering and Fuzzy C-mean Algorithm
Image segmentation, which is an important stage of many image processing algorithms, is the process of partitioning an image into nonintersecting regions, such that each region is homogeneous and the union of no two adjacent regions is homogeneous. This paper presents a novel pixon-based algorithm for image segmentation. The key idea is to create a pixon model by combining fuzzy filtering as a ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of medical signals and sensorsجلد ۵، شماره ۳، صفحات ۱۷۶-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023