analysis of colorectal cancer and polyps for the presence of herpes simplex virus and epstein - barr virus dna sequences by polymerase chain reaction
نویسندگان
چکیده
background: colorectal cancer is one of the most common malignancies worldwide with more than one million new cases diagnosed each year . the aim of this study is to investigate the prevalence of herpes simplex virus and epstein-barr virus in patients with colorectal carcinomas and polyps in comparison with healthy subjects by using the polymerase chain reaction technique. methods: in this analytical case-control study, we selected 15 patients with colorectal cancer , 20 patients with colorectal polyps and 35 patients without malignancy as controls. biopsy specimens were frozen under sterile conditions at -20ºc. after dna extraction, analysis of polymerase chain reaction to detect herpes simplex virus and epstein-barr virus dna in tissue samples was performed. statistical analysis was performed with the χ2 test. results: we observed herpes simplex dna in 33.3% of tumor samples (5 of 15) and 20% from the non-malignant control group (7 of 35). there was no herpes simplex dna in the polyp tissues (0 of 20). epstein-barr dna was found in 60% of tumor samples (9 of 15), 35% of polyp samples (7 of 20), and 40% of the non-malignant control group (14 of 35). statistical analysis showed no significant association between the prevalence of herpes simplex and epstein- barr viruses and the incidence of colorectal cancer and polyps compared with the control group. conclusion: the results demonstrate a lack of direct molecular evidence to support an association between herpes simplex and epstein-barr viruses with human colorectal malignancies. these results do not exclude a possible oncogenic role of these viruses to infect different colon cells.
منابع مشابه
Demonstration of Herpes Simplex Virus, Cytomegalovirus and Epstein-Barr Virus in Colorectal Cancer
Background: The present study sought to investigate molecular evidence for association between the presence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in CRC and colorectal polyp by using the PCR method in Iran. Methods: In this analytical case-control study, we selected 15 patients with CRC, 20 patients with colorectal polyp, and 35 patients without mali...
متن کاملAnalysis of colorectal cancer and polyp for presence herpes simplex virus and cytomegalovirus DNA sequences by polymerase chain reaction
Introduction Colorectal cancer (CRC) is the most common gastrointestinal cancer and the leading cause of cancer deaths in Iran.1 According to the World Health Organization (WHO), approximately, 875000 new cases of CRC are diagnosed annually worldwide.2 In general, the most CRCs are immunologically silent tumors, grow slowly and often do not produce symptoms until they reach a large size. The in...
متن کاملبررسی herpes simplex virus,epstein barr virus and cytomegalovirus در مایع شیار لثه ای بیماران مبتلا به پریو دنتیت مزمن به روش poly merase chain reaction.
چکیده بیماری های پریودونتال، از جمله بیماریهای شایع و مولتی فاکتوریال دهان است. نقش میکروب ها در اتیولوژی آن به خوبی شناسایی شده است. اما شواهد نشان می دهند که ویروس های مشخصی نیز در پاتوژنز آن نقش دارند. هدف از مطالعه ی حاضر، بررسی ویروس های hsv، ebv و cmv در مایع شیار لثه ای بیماران پریودنتیت مزمن و مقایسه ی آن با افراد سالم بود. مواد و روش کار: مایع شیار لثه ای از دو گروه از افراد شامل 3...
demonstration of herpes simplex virus, cytomegalovirus and epstein-barr virus in colorectal cancer
background: the present study sought to investigate molecular evidence for association between the presence of herpes simplex virus (hsv), cytomegalovirus (cmv), and epstein-barr virus (ebv) in crc and colorectal polyp by using the pcr method in iran. methods: in this analytical case-control study, we selected 15 patients with crc, 20 patients with colorectal polyp, and 35 patients without mali...
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عنوان ژورنال:
middle east journal of cancerجلد ۶، شماره ۴، صفحات ۲۱۱-۰
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