the effect of resveratrol on the expression of mdr1 gene in leukemic lymphoblast’s of acute lymphoblastic leukemia patients

نویسندگان

abbasali hoseinpoor feyzi hematology and oncology research center, tabriz university of medical sciences, tabriz, iran

majid farshdousti hagh hematology and oncology research center, tabriz university of medical sciences, tabriz, iran

tohid ebadi . department of hematology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

karim shams-asanjan . department of hematology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

چکیده

background: chemotherapy plays a very important role in the treatment of leukemia but the resistance properties of the lymphoblasts limit the effect of chemotherapy. one of the main mechanisms of resistance to chemotherapy is the increased expression of mdr1 gene. the aim of this study was to explore the effect of resveratrol on the expression of mdr1 gene in leukemic lymphoblast of new cases of acute lymphoblastic leukemia (all) patients in vitro. methods: separation of lymphoblasts of 5 new case all patients from peripheral blood was performed by ficoll density gradient centrifugation. lymphoblasts were cultured in rpmi 1640 medium. lymphoblasts were treated with 50µmol/l resveratrol for 48 h. total rna was extracted with guanidine isothiocyanate. rna was converted to cdna. real time pcr was used to detect mrna expression of mdr1. results: the results of gene detection showed that the expression of mdr1 did not change significantly in the patients however, in one patient expression of mdr1 increased upon treatment with resveratrol. conclusion: the results of this study did not support resveratrol as a compound to reverse multidrug resistance in leukemic lymphoblasts.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast’s of acute lymphoblastic leukemia patients

 Background: Chemotherapy plays a very important role in the treatment of leukemia but the resistance properties of the lymphoblasts limit the effect of chemotherapy. One of the main mechanisms of resistance to chemotherapy is the increased expression of MDR1 gene. The aim of this study was to explore the effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast of new...

متن کامل

the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...

the washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests

در این پایان نامه تاثیر دو نوع تست جزیی نگر و کلی نگر بر به یادسپاری محتوا ارزیابی شده که نتایج نشان دهندهکارایی تستهای کلی نگر بیشتر از سایر آزمونها است

15 صفحه اول

The effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast’s of acute lymphoblastic leukemia patients

BACKGROUND Chemotherapy plays a very important role in the treatment of leukemia but the resistance properties of the lymphoblasts limit the effect of chemotherapy. One of the main mechanisms of resistance to chemotherapy is the increased expression of MDR1 gene. The aim of this study was to explore the effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast of new cases of...

متن کامل

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

the effect of learning strategies on the speaking ability of iranian students in the context of language institutes

the effect of learning strategies on the speaking ability of iranian students in the context of language institutes abstract language learning strategies are of the most important factors that help language learners to learn a foreign language and how they can deal with the four language skills specifically speaking skill effectively. acknowledging the great impact of learning strategies...

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
caspian journal of internal medicine

جلد ۶، شماره ۲، صفحات ۱۱۳-۱۱۵

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023