expression, purification and immunogenic description of a hepatitis c virus recombinant coree1e2 protein expressed by yeast pichia pastoris

conclusions these findings of the present study indicate that the recombinant coree1e2 glycoprotein is effective in inducing neutralizing antibodies, and is an influential hcv vaccine candidate. results in our study, the virus-like particle of rcoree1e2 with 70 nm size, was shown by electron microscopy and proved the self-assembly in vitro in a yeast expression system. materials and methods by a codon optimization technique based on the p. pastoris expression system, we could increase the rate of recombinant proteins. moreover, the purified protein can efficiently induce anti-coree1e2 antibodies in rabbits, and also by developing a homemade enzyme-linked elisa kit we can detect antibody of hcv iranian patients with genotype 1a. objectives in this study, the pichia pastoris expression system was used to express a recombinant hcv coree1e2 protein, which consists of core (269 nt-841nt) e1 (842 nt-1417nt) and e2 (1418 nt-2506nt). background gradual development of a useful vaccine can be the main point in the control and eradication of hepatitis c virus (hcv) infection. hepatitis c virus envelope glycoproteins are considered as the main hcv vaccine candidate.