typing of hla class i by polymerase chain reaction-sequence specific oligonucleotide primer (pcr-ssop) technique in iranian cord blood donors

background: hla compatibility between transplant donor and recipient is one of the major determinants of transplant outcome. objective: to determine hla class i by pcr- sequence-specific oligonucleotide probe (pcr-ssop) in cord blood donors. methods: genomic dna of 142 cord blood samples registered at the cord blood bank of iran at hematology, oncology, and bone marrow transplantation research center, was prepared and hla class i was determined by the pcr-ssop. results: a total of 284 hla-a alleles was identified of which a*02 and a*24 were the most common. among 284 hla-b and hla-c alleles, b*35, b*51, cw*4 and cw*12 were the most frequent alleles in the studied population. conclusion: amplification of hla loci with pcr-ssop has proved to be a reliable method for hla-a, -b and -c genotyping.