effect of ganoderma lucidum on cytokine release by peritoneal macrophages
نویسندگان
چکیده
background: the water-soluble extract of ganoderma lucidum (reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. objective: to investigate the effect of ganoderma lucidum (g. lucidum) on cytokine production by mice peritoneal macrophages. methods: mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold pbs. peritoneal macrophages were plated out at 1x106 cell/well in 1ml rpmi 1640 medium supplemented with 10%fcs, 50 μg streptomycin and 50u penicillin. cells were incubated in the presence or absence of different concentrations of g. lucidum at 370c and 5% co2 for 48 hours. cell free medium was removed and used for cytokine assay by elisa method (bender med system). results: the results showed no significant differences in cell viability at concentrations ranged from 0-40 μg/ml compared with control group. g. lucidum enhanced il-1β, tnf-α and no production in a concentration dependent manner. however, it is not clear if the enhancement of no release is due to direct effect of g. lucidum on no synthesis or by indirect endogenous modulation via cytokines. il-12 release by peritoneal macrophages was also increased in response to different concentrations of g. lucidum, but maximum enhancement was induced in response to 5 μg/ml of g. lucidum (p
منابع مشابه
Effect of Ganoderma Lucidum on Cytokine Release by Peritoneal Macrophages
Background: The water-soluble extract of Ganoderma lucidum (Reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. Objective: To investigate the effect of Ganoderma lucidum (G. lucidum) on cytokine production by mice peritoneal macrophages. Methods: Mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold PBS. Per...
متن کاملEffect of Ganoderma lucidum on cytokine release by peritoneal macrophages.
BACKGROUND The water-soluble extract of Ganoderma lucidum (Reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. OBJECTIVES To investigate the effect of Ganoderma lucidum (G. lucidum) on cytokine production by mice peritoneal macrophages. METHODS Mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold PBS. P...
متن کاملEffect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets
Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containi...
متن کاملEFFECT OF FIVE ALPHA DIHYDROTESTOSTERONE (5α-DHT) ON CYTOKINE PRODUCTION BY PERITONEAL MACROPHAGES OF NZB/BALBc MICE
One of the mechanisms involved in the regulation of the immune system by steroid hormones could be the monocytic-macrophage system. In this study the effect of the male hormone 5a-DHT on cytokine release by peritoneal macrophages (mΦ) of male and female NZB/BALBc mice was investigated. Macrophages from male mice activated with LPS produced a greater amount of IL-1β (21.8%) (p<0.05) and IL-...
متن کاملEffect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets
OBJECTIVE Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level. This study was undertaken to investigate its direct effect on the islets of Langerhans. MATERIAL AND METHODS Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer contain...
متن کاملEffects of T-2 Toxin on Cytokine Produc-tion by Mice Peritoneal Macrophages and Lymph Node T-Cells
Background: T-2 toxin is a mycotoxin of type A trichothecenes produced by several fungal genera such as Fusarium species. Mycotoxins can affect both cell mediated and humoral immune compartments. Objective: The purpose of this study was to investi-gate the effect of T-2 toxin on cytokine production by mouse peritoneal macrophages and lymph node T cells. Methods: Mouse peritoneal macrophages and...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of immunologyجلد ۴، شماره ۴، صفحات ۲۲۰-۲۲۶
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023