development and evaluation of tums medium, a novel biphasic culture medium for isolation of brucella spp. from patients
نویسندگان
چکیده
background and objectives : there are limitations in time and technique for isolation of brucella from patients. we developed a new brucella culture medium and evaluated its efficiency compared to bactec blood culture system and serology. materials and methods: a bi-phasic medium containing urea agar and brain heart infusion was formulated. appearance of clear red color in liquid phase was the basis of positivity for brucella. the new medium which is designated as tums medium (tums refers to tehran university of medical sciences) and bactec blood culture vials were inoculated with different concentrations of 20 brucella strains. the blood samples from 58 suspected patients were tested by both media and serology (wright and coombs). any growth was sub-cultured and suspected colonies were identified by standard methods. results: the tums medium detected more positive samples (100%) than bactec (85%) when the organism was suspended at lower concentration (10 cfu). of 58 blood cultures, 47 (81%) samples tested on tums medium (incubation period =4.2 days) and 39 (67.2 %) samples tested on bactec (incubation period =3.3 days) were found positive. conclusion: the tums medium was superior to others in detecting the organism from patients with clinical signs or who took medications for >1year. the tums medium is easy to prepare and use in endemic areas where resources are limited.
منابع مشابه
Evaluation of a Novel Biphasic Culture Medium for Recovery of Mycobacteria: A Multi-Center Study
BACKGROUND Mycobacterial culture and identification provide a definitive diagnosis of TB. Culture on Löwenstein-Jensen (L-J) medium is invariably delayed because of the slow growth of M. tuberculosis on L-J slants. Automated liquid culture systems are expensive. A low-cost culturing medium capable of rapidly indicating the presence of mycobacteria is needed. The aim of this study was to develop...
متن کاملEVALUATION OF TWO BIPHASIC CULTURE MEDIA FOR ISOLATION OF LEISHMANIA PARASITES FROM CUTANEOUS ULCERS IN IRANIAN PATIENTS
متن کامل
wuthering heights and the concept of marality/a sociological study of the novel
to discuss my point, i have collected quite a number of articles, anthologies, and books about "wuthering heights" applying various ideas and theories to this fantastic story. hence, i have come to believe that gadamer and jauss are rightful when they claim that "the individaul human mind is the center and origin of all meaning," 3 that reading literature is a reader-oriented activity, that it ...
15 صفحه اولSelective medium for the isolation and enumeration of Klebsiella spp.
A highly selective medium for the enumeration and isolation of Klebsiella pneumoniae and Klebsiella oxytoca was developed in which the typical colonies were convex and 1 to 2 mm in diameter. Their pigment was either a mucoid pink-red color or a more watery pale red with a dark red center. Relatively little colonial growth occurred for any other bacterial genera, and where such colonies did grow...
متن کاملSelective medium for isolation and enumeration of Bifidobacterium spp.
A new method was developed for the isolation and enumeration of Bifidobacterium spp. from natural aquatic environments. The method was based on the utilization of a new medium, Bifidobacterium iodoacetate medium 25, and resuscitation techniques were used to isolate injured bifidobacteria. The new medium was tested with a nonselective reference medium on sewage and sewage-polluted surface waters...
متن کاملDevelopment of Culture Medium for the Isolation of Flavobacterium and Chryseobacterium from Rhizosphere Soil
An effective medium designated phosphate separately autoclaved Reasoner's 2A supplemented with cycloheximide and tobramycin (PSR2A-C/T) has been developed for the isolation of Flavobacterium and Chryseobacterium strains from the plant rhizosphere. It consists of Reasoner's 2A agar (R2A) prepared by autoclaving phosphate and agar separately and supplementing with 50 mg L(-1) cycloheximide and 1 ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of microbiologyجلد ۱، شماره ۲، صفحات ۲۱-۲۵
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023