a model to study the phenotypic changes of insect cell transfection by copepod super green fluorescent protein (cop-gfp) in baculovirus expression system

background: baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. the presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. methods: the aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copgfp). in this light, the resultant vector was constructed and used for transfection of spodoptera frugiperda cells. results: expression of the copgfp protein in insect cells was confirmed by fluorescent microscopy and western-blot analysis. conclusion: the application of copgfp control bacmid can be considered as an appropriate control for insect cell transfection.