caspase inhibition in neuroinflammation induced by soluble β amyloid monomer, protects cells from abnormal survival and proliferation, via attenuation of nfқb activity
نویسندگان
چکیده
introduction: evidence suggests that neuronal apoptosis in neurodegenerative diseases is correlated with inflammatory reactions. the beneficial or detrimental role of apoptosis in neuroinflammation is unclear. elucidating this question may be helpful in management of neurodegenerative diseases. since tnf-α is able to induce apoptosis as well as increased viability of the cells by activation of caspases or nf-kb, respectively, the question is what will happen if the balance between the two pathways is disturbed by inhibition of apoptosis. methods: in this study, we used β–amyloid peptide (soluble aβ monomer) injection into the wistar male rat prefrontal cortex for induction of neuroinflammation in the hippocampus. levels of tnf-α and caspase-3 were determined via western blot analysis. using chronic intracerebroventricular administration of caspase inhibitors, z-vad –fmk and z-devd-fmk, we inhibited apoptosis. exploring consequences of apoptosis inhibition, activity of nf-kb was evaluated via western blotting. results: after β–amyloid peptide injection we observed an increase in tnf-α and caspase3 as an inflammatory cytokine and apoptotic marker, respectively (p<0.001 and p<0.0001, respectively). as a consequences of apoptosis inhibition, nuclear nf-κb was decreased and cytosolic nf-κb was increased and these changes were significant compared to aβ-injected group (p<0.001 and p<0.05, respectively). conclusion: caspase inhibition as an initiator of apoptosis, probably by attenuation of nf-kb activity, protect cells from abnormal survival and proliferation.
منابع مشابه
Caspase inhibition in neuroinflammation induced by soluble β amyloid monomer, protects cells from abnormal survival and proliferation, via attenuation of NFқB activity
Introduction: Evidence suggests that neuronal apoptosis in neurodegenerative diseases is correlated with inflammatory reactions. The beneficial or detrimental role of apoptosis in neuroinflammation is unclear. Elucidating this question may be helpful in management of neurodegenerative diseases. Since TNF-α is able to induce apoptosis as well as increased viability of the cells by activation ...
متن کاملCaspase inhibition in neuroinflammation induced by soluble β amyloid monomer, protects cells from abnormal survival and proliferation, via attenuation of NFқB activity
Introduction: Evidence suggests that neuronal apoptosis in neurodegenerative diseases is correlated with inflammatory reactions. The beneficial or detrimental role of apoptosis in neuroinflammation is unclear. Elucidating this question may be helpful in management of neurodegenerative diseases. Since TNF-α is able to induce apoptosis as well as increased viability of the cells by activation of ...
متن کاملAttenuation of β-amyloid-induced neuroinflammation by KHG21834 in vivo
Eun-A Kim, Hoh-Gyu Hahn, Tae Ue Kim, Soo Young Choi & Sung-Woo Cho* Departments of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, Biomedical Laboratory Science, Yonsei University, Wonju 222-701, Life Sciences, Korea Institute of Science and Technology, Seoul 136-791, Biomedical Sciences and Research Institute for Bioscience and Biotechnology, Hallym ...
متن کاملThiorphan-induced survival and proliferation of rat thymocytes by activation of Akt/survivin pathway and inhibition of caspase-3 activity.
The activity of substance P (SP) in the rat thymus seems to be tightly controlled by its bioavailability. In this study, we provide evidence for the expression of the SP-degrading enzyme, neutral endopeptidase (NEP)/CD10, by rat thymocyte subsets, and we illustrate its involvement in the in vivo SP/neurokinin-1 receptor (NK(1)R)-mediated regulation of thymocyte survival and proliferation. NEP/C...
متن کاملA role for cyclooxygenase-1 in β-amyloid-induced neuroinflammation
(AD) is a chronic neuro-degenerative disorder characterized by progressive cognitive decline and memory loss. Accumulation of β-amyloid (Aβ) and tau protein are believed to be important pathological features of AD [1]. Results from a large number of studies suggest that neuro-inflammation is a key contributor to neuronal loss in AD. Anti-inflammatory drugs, in particular non-steroidal anti-infl...
متن کاملTemplate induced conformational change of amyloid-β monomer.
Population of aggregation-prone conformers for the monomeric amyloid-β (Aβ) can dramatically speed up its fibrillar aggregation. In this work, we study the effect of preformed template on the conformational distributions of the monomeric Aβ by replica exchange molecular dynamics. Our results show that the template consisting of Aβ peptides with cross-β structure can induce the formation of β-ri...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
physiology and pharmacologyجلد ۱۴، شماره ۳، صفحات ۲۱۱-۲۱۹
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023