radiographic & histopathological analysis in calvarias bone regeneration process by platelet-rich plasma, platelet-rich plasma–gel and auto bone chips in rat

a functional treatment for skeletal damages in orthopedic and oral maxillofacial surgeries is required. platelet growth factors such as platelet derived growth factor (pdgf), bone morphogenic factor (bmp), transferring growth factor-β (tgf-β) and insulin-like growth factor-1 (igf-1) proceed wound healing and bone regeneration. in the present study we focused on the effect of platelet rich plasma (prp), platelet rich plasma gel (prp-gel) and auto bone chips on this process. 30 male, 22 weeks old, sprague-dawley rats weighing 525 g were used. they were divided in three groups consisting of prp (treated by platelet-rich plasma), prp-gel (treated by it), bone chips and control (two cavities created in each animal in this group). after 16 weeks they were histologically investigated while in the periods of 40, 60, 90and 120 days, the radiography had been done. the radiographic analysis showed complete treatment in all groups; however, by the histo-pathological investigations by auto bone chips complete and prp-gel partial healing has been observed. by histo-morphometric surveys (100±25) % in bone chips and (50±25) % in prp-gel groups bone bridging were observed, whereas in prp it was not noticeable. the present study suggests that neither prp, nor prp-gel could be as beneficial as bone chips. statistically, in prp-gel group, due to the existence of fibrin and thrombin, solid bone bridging at the treated site is indicated. according to the previous studies, in which the key role of both inhibitory and stimulatory signals in controlling the bone regeneration were proven, we suggest that auto bone chips could completely enhance healing due to signals among blood factors, environmental tissues and skeletal particles.