reverse staining method of polyacrylamide gels by imidazole-zinc salts for

Comparative advantages of imidazole-sodium dodecyl sulfate-zinc reverse staining in polyacrylamide gels.

Polyacrylamide gel electrophoresis (PAGE), which is widely used to assess protein purity and molecular weight [1], is best combined with a highly sensitive, reproducible, inexpensive, rapid, and easy to perform protein staining/detection method. Coomassie brilliant blue (CBB)1 staining is a popular method, known for its simplicity, economy, and compatibility with downstream analysis but relativ...

An effective method for silver-staining DNA in large numbers of polyacrylamide gels.

Silver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR-SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming ...

Silver-staining of proteins in polyacrylamide gels: a general overview.

On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including standard methods for unsupported gels and new methods devised for thin (0....

Agarose overlays allow simplified staining of polyacrylamide gels.

Laser scanners from Molecular Dynamics (Sunnyvale, CA, USA) or Hitachi Scientific Instruments (Mountain View, CA, USA) are being used increasingly to detect DNA in polyacrylamide gels. The standard technique for visualizing DNA is to separate the plates of a gel and pour a diluted nucleic acid stain such as ethidium bromide or SYBR Green I [from Molecular Probes (Eugene, OR, USA); also markete...

Rapid Coomassie blue staining and destaining of polyacrylamide gels.

Visible fluorescent detection of proteins in polyacrylamide gels without staining.

2,2,2-Trichloroethanol (TCE) incorporated into polyacrylamide gels before polymerization provides fluorescent visible detection of proteins in less than 5min of total processing time. The tryptophans in proteins undergo an ultraviolet light-induced reaction with trihalocompounds to produce fluorescence in the visible range so that the protein bands can be visualized on a 300-nm transilluminator...