standardization of an indirect elisa for serological diagnosis of aspergilloma

نویسندگان

nariman shah-hosseini islamic azad university, lahijan branch, faculty of science, department of microbiology, lahijan, ir iran; islamic azad university, lahijan branch, faculty of science, department of microbiology, lahijan, ir iran. tel.: +98-1412229081, fax: +98-1412229081

alireza khabiri pasteur institute of iran, department of mycology, tehran, ir iran

farideh bagheri pasteur institute of iran, department of mycology, tehran, ir iran

hashem yazdi pasteur institute of iran, department of mycology, tehran, ir iran

چکیده

conclusions: it was concluded that the elisa is a suitable method for large scale screening of antibodies against aspergilloma. the easy techniques have shortened the required time of tests (approximately 1.30 hours for our elisa, as opposed to 2.30 hours with commercial elisa kit). this may be a substantial benefit for large numbers of samples that should be tested. materials and methods: an indirect enzyme linked immunoassay (elisa) was developed and standardized for the serological diagnosis of aspergilloma. results: among 500 serum samples, 35 had positive and 465had negative elisa test against aspergilloma. in comparison, serum samples were tested by immunoblotting test, considered as the standard. in relation to the immunoblotting, the elisa presented 94.2% sensitivity, 99.5% specificity, 94.2% positive predictive value, 99.5% negative predictive value and 99.2% precision. background: so far several literatures have discussed that gmp as a major cell wall component in aspergillus species and can also be secreted into the medium as a component of the exoantigen. moreover, one of the critical points in designing an elisa is the preparation of the antigen. objectives: the main aim of this study was the identification of gmp and standardization of an indirect elisa for the serological diagnosis of aspergilloma.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5.

Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of inf...

متن کامل

Enzootic Bovine Leukosis: Performance of an Indirect Elisa Applied in Serological Diagnosis

Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented....

متن کامل

Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain

Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L. infantum) (Mediterranean type) and its major reservoir host is the dog. Ob-jective: To compare the serological methods including direct agglutination test (DAT), indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent as-say (ELISA) for serodiagnosis of endemic strain of L. i...

متن کامل

an application of fuzzy logic for car insurance underwriting

در ایران بیمه خودرو سهم بزرگی در صنعت بیمه دارد. تعیین حق بیمه مناسب و عادلانه نیازمند طبقه بندی خریداران بیمه نامه براساس خطرات احتمالی آنها است. عوامل ریسکی فراوانی می تواند بر این قیمت گذاری تاثیر بگذارد. طبقه بندی و تعیین میزان تاثیر گذاری هر عامل ریسکی بر قیمت گذاری بیمه خودرو پیچیدگی خاصی دارد. در این پایان نامه سعی در ارائه راهی جدید برای طبقه بندی عوامل ریسکی با استفاده از اصول و روش ها...

A Comparative Study of an Elisa Test and an Indirect Immunofluorescence Test for Serological Diagnosis of Babesia Bovis Infection

A COMPARATIVE STUDY OF AN ELISA TEST AND AN INDIRECT IMMUNOFLUORESCENCE TEST FOR SEROLOGICAL DIAGNOSIS OF BABESIA BOVIS INFECTION. Detection of antibodies to Babesia bovis in cattle is essential for the understanding of the epidemiology of babesiosis and this study was concerned with comparing the indirect fluorescent antibody with the ELISA. Both assays gave rise to 100% sensitivity whilst the...

متن کامل

Diagnosis of mixed gastrointestinal nematode infection in goat by an indirect-ELISA

An indirect-ELISA for the diagnosis of mixed gastrointestinal (GI) nematode infection comprising Oesophagostomum, Haemonchus and Trichuris species was standardized using crude somatic antigen of Oesophagostomum columbianum (CSAg-Oc) and sera of slaughtered goats with known parasitological status including Oesophagostomum, Haemonchus, and <em...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
archives of clinical infectious diseases

جلد ۷، شماره ۳، صفحات ۹۲-۵

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023