efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model
نویسندگان
چکیده
background: although cervical cancer mortality has reduced during last years, but it is still leading cause of mortality among women. many efforts have performed to develop new drugs and strategy to cure cervical cancer. mesenchymal stem cells (mscs) have many advantages that make them a suitable choice as a cell therapeutic agent in cancer treatment. there are different transfection methods for mscs transduction that one of them is based on application of lentiviral vector. in this study we aimed to develop an improved protocol for mouse mscs transduction as well as assess the homing capacity and incorporation of mscs in cervical cancer model. methods : mscs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. in our study, lenti vector transductions of mscs performed by three times inoculation of virus with interval of eight hours. their penetrations were detected in tissue sections after injection of transduced mscs to cervical cancer model. results: the results showed that, mscs efficiently transduced with lenti vector using our improved protocol and have good penetration in tumor tissue. conclusion: the results provide evidence that, mscs were able to penetrate through tumor mass of cervical tumor model and are good vehicle for gene transfer to cervical cancer. please cite this article as: kenarkoohi a, soleimani m, bamdad t, soleimanjahi h, estiri h, razavi-nikoo h. efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model. iran j cancer prev. 2014;7(4):225-31.
منابع مشابه
Efficient Lentiviral Transduction of Adipose Tissue-Derived Mouse Mesenchymal Stem Cells and Assessment of Their Penetration in Female Mice Cervical Tumor Model
BACKGROUND Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we ...
متن کاملEvaluating the Viability of Adipose Tissue-derived Mesenchymal Stem Cells in Diabetic Culture Media Model
Introduction: In diabetes mellitus, stem cells are exposed to inappropriate conditions such as an increase of glucose in extracellular space, oxidative stress, and deprivation from growth factors (following ischemia). This study was aimed to evaluate the effect of these conditions on stem cells. Methods: Stem cells were isolated from subcutaneous adipose tissue of rats. The cells were maintaine...
متن کاملComparison of Characteristics of Human Amniotic Membrane and Human Adipose Tissue Derived Mesenchymal Stem Cells
BACKGROUND Mesenchymal stem cells (MSCs) are ideal candidates for treatment of diseases. Amniotic membranes are an inexpensive source of MSCs (AM-MSC) without any donor site morbidity in cell therapy. Adipose tissue derived stem cells (ASCs) are also suitable cells for cell therapy. There is discrepancy in CD271 expression among MSCs from different sources. In this study, the characteristics...
متن کاملCytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells
متن کامل
Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells
Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human A...
متن کاملSpermatogenesis after transplantation of adipose tissue-derived mesenchymal stem cells in busulfan-induced azoospermic hamster
Objective(s): Adipose tissue-derived mesenchymal stem cells (AT-MSCs) with more potent immunomodulatory effects, greater proliferative potential and secretion of growth factors and cytokines in comparison with bone marrow derived MSCs are more appropriate for cell therapy. The aims of the present study were to evaluate the histomorphometric effect of AT-MSCs allotransplantation on regeneration ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of cancer preventionجلد ۷، شماره ۴، صفحات ۲۲۵-۳۱
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023