comparison of elisa and ifa for estimation of antibody levels of cattle to theileria annulata vaccine

نویسندگان

fariba golcliiiifar

it. mndani

r. haslicmi-fcsharakr

k. esmailnya

چکیده

bovine tropical theileriosis caused by theileria annulata is an economically important disease of cattle. an enzyme linked immunosorbent assay (elisa) was used to determine antibody levels in vaccinated and unvaccinated cattle, us¬ing cellular schizont as antigen and its results were compared with immunofluo-rescent assay (ifa). for this test 126 sera collected (105 vaccinated, 31 not vacci¬nated) from cows and assayed with elisa which among them 104 sera were positive and 32 sera were negative. same sample assayed with ifa in which 99 were positive sera and 37 were negative sera. thereby the sensitivity and speci¬ficity of this elisa on comparsion with ifa were 95.5% and 66.6% respectively. this study revealed that elisa could be successfully used for both differentiat¬ing vaccinated and not vaccinated cattle and obtaining the titer of vaccinated cattle.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparative evaluation of antibody positive titer by ELISA and IFA in Theileria annulata vaccinated cattle in Iran.

An enzyme linked immunosorbent assay (ELISA) was used to evaluate antibody positive titer in vaccinated and non-vaccinated cattle using schizont infected myeloid cells as an antigen. The result was compared with indirect fluorescent antibody level in the same animals. For this study 116 milking cows, 95 vaccinated and 21 non-vaccinated, were bleeded in order to prepare sera. They were tested wi...

متن کامل

the innovation of a statistical model to estimate dependable rainfall (dr) and develop it for determination and classification of drought and wet years of iran

آب حاصل از بارش منبع تأمین نیازهای بی شمار جانداران به ویژه انسان است و هرگونه کاهش در کم و کیف آن مستقیماً حیات موجودات زنده را تحت تأثیر منفی قرار می دهد. نوسان سال به سال بارش از ویژگی های اساسی و بسیار مهم بارش های سالانه ایران محسوب می شود که آثار زیان بار آن در تمام عرصه های اقتصادی، اجتماعی و حتی سیاسی- امنیتی به نحوی منعکس می شود. چون میزان آب ناشی از بارش یکی از مولفه های اصلی برنامه ...

15 صفحه اول

a comparison of teachers and supervisors, with respect to teacher efficacy and reflection

supervisors play an undeniable role in training teachers, before starting their professional experience by preparing them, at the initial years of their teaching by checking their work within the proper framework, and later on during their teaching by assessing their progress. but surprisingly, exploring their attributes, professional demands, and qualifications has remained a neglected theme i...

15 صفحه اول

the evaluation and comparison of two esp textbooks available on the iranian market for teaching english to the students of medicine

abstract this study evaluated and compared medical terminology and english for the students of medicine (ii) as two representatives of the textbooks available on the iranian market for teaching english to the students of medicine. this research was performed on the basis of a teacher’s and a number of students’ attitudes and the students’ needs analysis for two reasons: first, to investigate...

15 صفحه اول

a comparison of linguistic and pragmatic knowledge: a case of iranian learners of english

در این تحقیق دانش زبانشناسی و کاربردشناسی زبان آموزان ایرانی در سطح بالای متوسط مقایسه شد. 50 دانش آموز با سابقه آموزشی مشابه از شش آموزشگاه زبان مختلف در دو آزمون دانش زبانشناسی و آزمون دانش گفتار شناسی زبان انگلیسی شرکت کردند که سوالات هر دو تست توسط محقق تهیه شده بود. همچنین در این تحقیق کارایی کتابهای آموزشی زبان در فراهم آوردن درون داد کافی برای زبان آموزان ایرانی به عنوان هدف جانبی تحقیق ...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of allergy, asthma and immunology

جلد ۲، شماره ۲، صفحات ۱۰۱-۱۰۵

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023