response of human t cells to tetanus neurotoxin hcc sub-domain: t cell cytokine production and activation marker induced by hcc
نویسندگان
چکیده
tetanus is caused by the tetanus neurotoxin (tent), a 150 kda single polypeptide molecule which is cleaved into active two-chain molecules composed of a 50 kda n-terminal light (l) and a 100 kda c-terminal heavy (h) chains. fragment c is further subdivided into two subdomains: the proximal hcn subdomain and the extreme carboxy subdomain, hcc. hcc is considered as an immunodominant part of tent and is responsible for tent binding activity to neurons.in the present study, we investigated the ability of recombinant hcc(r hcc) to induce t cell activation. our results showed that recombinant hcc has a stimulatory effect on ifn-γ secretion by t cells after 48h co-incubation in the presence of anti-tlr-2 ab. also, hcc can induce the expression of cd69 on t cells.our finding indicated that stimulatory effects of hcc on t cells are tlr-2 independent and anti-tlr-2 inhibitory antibody fails to neutralize hcc stimulatory effects on t cells.furthermore, hcc is critical for immunogenic activity of tent and is able to induce t cells through tlr-2 independent pathway.
منابع مشابه
Response of Human T Cells to Tetanus Neurotoxin HCC Sub-Domain: T Cell Cytokine Production and Activation Marker Induced by HCC.
Tetanus is caused by the tetanus neurotoxin (TeNT), a 150 kDa single polypeptide molecule which is cleaved into active two-chain molecules composed of a 50 kDa N-terminal light (L) and a 100 kDa C-terminal heavy (H) chains. Fragment C is further subdivided into two subdomains: the proximal HCN subdomain and the extreme carboxy subdomain, HCC. HCC is considered as an immunodominant part of TeNT...
متن کاملLoss of butyrate-induced apoptosis in human hepatoma cell lines HCC-M and HCC-T having substantial Bcl-2 expression.
We have demonstrated that sodium butyrate induces differentiation in human hepatoma cells; however, recent studies have shown that this agent causes apoptosis in some types of cancer cells. In this study, we examined whether sodium butyrate causes apoptosis in the human hepatoma cell lines, HCC-M and HCC-T. The growth of human hepatoma cells was dose-dependently reduced by sodium butyrate. Flow...
متن کاملProfile of interleukin-2 production in human mononuclear cells and T-cell lines
Introduction: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role Background and Objective: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role in activation, growth and differentiation of several immune cells. Moreover IL-2 is known as a pro-inflammatory and anti-tumoral cytokine. In addition deysregulation of IL-2 in some diseases such as ...
متن کاملaltered mitochondrial response to activation of t-cells in neonate
mitochondrial functions have a major impact on t-cell functionality. in this study we characterized whether mitochondrial function in the neonatal t-cells differs from that in the adult t-cells during short t-cell activation. methods: we used fow cytometry methods to test mitochondrial mass and to monitor mitochondrial ca2+ levels, mitochondrial potential and superoxide generation in parallel w...
متن کاملFibroblast and T cells conditioned media induce maturation dendritic cell and promote T helper immune response
Dendritic cells (DCs) induce pathogen-specific T cell responses. We comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), PHA activated T cells (T cell conditioned medium), and mixture of fibroblast & PHA activated T cells (FCM-TCCM) conditioned media on maturation of DCs. Monocytes were cultured with GM-CSF and IL-4 for five days. ...
متن کاملThe Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells
Background: Dendritic cells (DC) are a key regulator of the immune response, and interferon- beta (IFN-β) is considered an immunomodulatory molecule for DC. Objective: The purpose of this study was to evaluate the ability of IFN-β treated DC to induce cytokine secretion by CD4+ T cells. Methods: Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating fac...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۴، شماره ۵، صفحات ۵۱۹-۵۲۵
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023