putative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (holothuria leucospilota) as an antioxidant compound
نویسندگان
چکیده
objective(s):marine organisms are known as a potential source of natural products, which contain bioactive substances with therapeutic properties. sea cucumbers are prominent among marine organisms because of their dietary and therapeutic applications. in addition, they have capacity of synthesizing saponins molecules and other metabolites with therapeutic properties such as antitumor, antimicrobial, anti-inflammatory and antioxidant activities. the aim of this study was to evaluate the antioxidant and pro-apoptotic effects of sea cucumber saponins (scs) isolated from holothuria leucospilota species. materials and methods: evaluation of antioxidant activity of scs was carried out by dpph (1, 1-diphenyl-2-picrylhydrazyl), abts (azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), power reducing and total antioxidant assays. the anti-proliferative effect was studied by мтт (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. mechanisms leading to apoptosis were also evaluated byfluorescence microscopy, flow cytometry and real time pcr. results: the results showed that the dpph and abts activities increased in a dose dependent manner. the reducing capacity enhanced with increasing concentration of the saponin extract (0 to 2 mg/ml). the scs exhibited moderate total antioxidant activity. evaluation of anti-proliferative effect revealed that scs with ic50 of about 6 μg/ml, can display a good cytotoxic activity in a dose dependent manner. further apoptosis induction was confirmed by fluorescence microscopy and flow cytometry. sea cucumber saponin was also found to exert a pro-apoptotic effect by increasing the expression of bax and decreasing the expression of bcl2. conclusion: these results indicate that the scs may act as a natural antioxidant and antitumor agent.
منابع مشابه
Putative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (Holothuria leucospilota) as an antioxidant compound
Objective(s):Marine organisms are known as a potential source of natural products, which contain bioactive substances with therapeutic properties. Sea cucumbers are prominent among marine organisms because of their dietary and therapeutic applications. In addition, they have capacity of synthesizing saponins molecules and other metabolites with therapeutic properties such as antitumor, antimicr...
متن کاملPutative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (Holothuria leucospilota) as an antioxidant compound
OBJECTIVES Marine organisms are known as a potential source of natural products, which contain bioactive substances with therapeutic properties. Sea cucumbers are prominent among marine organisms because of their dietary and therapeutic applications. In addition, they have capacity of synthesizing saponins molecules and other metabolites with therapeutic properties such as antitumor, antimicrob...
متن کاملHemolytic and Cytotoxic Properties of Saponin Purified from Holothuria leucospilota Sea Cucumber
Background: Holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the presence o...
متن کاملTranscriptional Analysis of VEGF-D and TGFβ Genes in MCF7 Cells Exposed to Saponin Isolated from Holothuria leucospilota (Sea Cucumber)
Background: Marine natural products contain a wide range of bioactive compounds with therapeutic properties that have revealed crucial properties in the treatment of some diseases. Some of these compounds have recently received considerable attention for drug discovery. In this study we examined the anti-angiogenic effect of saponin isolated from Holothuria leucospilota (sea cucumber) through e...
متن کاملTranscriptional analysis of VEGF-D and TGFβ genes in MCF7 cells exposed to saponin isolated from Holothuria leucospilota (sea cucumber).
BACKGROUND Marine natural products contain a wide range of bioactive compounds with therapeutic properties that have revealed crucial properties in the treatment of some diseases. Some of these compounds have recently received considerable attention for drug discovery. In this study we examined the anti-angiogenic effect of saponin isolated from Holothuria leucospilota (sea cucumber) through ev...
متن کاملhemolytic and cytotoxic properties of saponin purified from holothuria leucospilota sea cucumber
background: holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. saponins exhibit a wide spectrum of pharmacological and biological activities. in this study, we isolated the crude saponins from the body wall of the dominant iranian species of sea cucumber, holothuria leucospilota (h. leucospilota). the purpose of this study was to confirm the presence o...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۸، شماره ۲، صفحات ۱۸۰-۱۸۷
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023