protective effect of bioactive compounds from lonicera japonica thunb. against h2o2-induced cytotoxicity using neonatal rat cardiomyocytes

objective(s):pharmacological studies showed that the extracts of jin yin hua and its active constituents have lipid lowering, antipyretic, hepatoprotective, cytoprotective, antimicrobial, antibiotic, antioxidative, antiviral, and anti-inflammatory effects. the purpose of the present study was to investigate the protective effects of caffeoylquinic acids (cqas) from jin yin hua against hydrogen peroxide (h2o2)-induced and hypoxia-induced cytotoxicity using neonatal rat cardiomyocytes. materials and methods:seven cqas (c1 to c7) isolated and identified from jin yin hua were used to examine the effects of h2o2-induced and hypoxia-induced cytotoxicity. we studied c4 and c6 as preventative bioactive compounds of the reactive oxygen species (ros) production, apoptotic pathway, and apoptosis-related gene expression. results:c4 and c6 were screened as bioactive compounds to exert a cytoprotective effect against oxidative injury. pretreatment with c4 and c6, dose-dependently attenuated hypoxia-induced ros production and reduced the ratio of gssg/gstotal. western blot data revealed that the inhibitory effect of c4 on h2o2-induced up and down-regulation of bcl-2, bax, caspase-3, and cleaved caspase-3. apoptosis was evaluated by detection of dna fragmentation using tunel assay, and quantified with annexin v/pi staining. conclusion: in vitro experiments revealed that both c4 and c6 protect cardiomyocytes from necrosis and apoptosis during h2o2-induced injury, via inhibiting the generation of ros and activation of caspase-3 apoptotic pathway. these results demonstrated that cqas might be a class of compounds which possess potent myocardial protective activity against the ischemic heart diseases related to oxidative stress.