purification of lipid transfer protein 2 (ltp2) from iranian rice paddy

Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2.

Purified lipid transfer protein LTP2 from barley applied on tobacco leaves eliminated symptoms caused by infiltration of Pseudomonas syringae pv. tabaci 153. Growth of the pathogen in leaves of transgenic tobacco plants was retarded when compared with non-transformed controls. The percentage of inoculation points that showed necrotic lesions was greatly reduced in transgenic tobacco (17-38% ver...

Gaseous emissions from flooded rice paddy agriculture

[1] Modification of continental land for agricultural use has increased over the last century. Atmospheric impact of this land use change has only been addressed for a few ecosystems and compounds. This paper provides, to date, the most comprehensive examination of gaseous emissions from rice paddies. We report seasonal emission ranges and integrated emission totals for 55 chemical species. Thi...

Antifungal activity of recombinant rice LTP2 on some phytopathogenic fungi

Plant lipid transfer proteins (LTPs) are members of the pathogenesis-related proteins (PR-14) and some of them exhibit activity against phytopathogenic fungi. To investigate whether rice LTP2 plays a role in antifungal activity, the coding region of an Iranian rice Ltp2 gene was cloned into expression vector pET24-d(+) and then expressed in Escherichia coli Rosetta strain (DE3). The potential a...

Purification and characterization of lipid transfer protein(s) from human lipoprotein-deficient plasma.

Lipid transfer activities from human plasma have been characterized to determine whether triglyceride and cholesteryl ester transfer proteins are identical. After sequential purification by phenyl-Sepharose, CM-cellulose, chromatofocusing, and gel filtration, both triglyceride and cholesteryl ester transfer activities were purified approximately 15,000-fold compared to lipoprotein-deficient pla...

Purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from Coffea canephora seeds.

Coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. Antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. We isolated and immunolocalized Cc-LTP2, a new lipid transfer protein (LTP) from Coffea canephora seeds. We report its antimicrobial activity again...

Protein purification from whole tissues . Fatemeh Mehrpooyan*