plant regeneration via somatic embryogenesis and organogenesis in verbascum speciosum schard
نویسندگان
چکیده
plant regeneration was achieved in verbascum speciosum schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. two types of calli, embryogenic and non-embryogenic, were induced from mature embryo explants on murashige and skoog (ms) medium supplemented with different concentrations of benzyl adenine (ba) and α-naphthalene acetic acid (naa). in order to further proliferate the somatic embryoids, the yellow and friable embryogenic calli were transferred on ms medium containing 0.5 mg-1 charchol and 0.1 or 1 mg-1 2,4-dichlorophenoxy acetic acid (2,4-d) or into ms medium containing 60 g-1 sucrose, 50 mgl-1 casein hydrolysate (ch), 0.5 mg-1 kinetin (kin), 5 mg-1 2,4-d and 0.5 mg-1 charchol. among the 3 tested media, ms medium containing 0.1 mg-1 2,4-d and 0.5 mg-1 charchol was more effective for proliferation of embryonic calli. somatic embryos were transferred to hormone free ms medium for maturation and shoot regeneration. in addition, shoots and roots regenerated from non-embryogenic calli in hormone free ms medium or containing naa and ba. shoot buds were obtained from non-embryogenic calli and they were transferred to ms medium supplemented with 1 mg-1 ba or kin for further growth and multiplication. regenerated plants then were potted and maintained in the greenhouse.
منابع مشابه
Somatic Embryogenesis and Plant Regeneration in Cucumber
The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars was examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and ‘Fushinarimidori’ produced the highest number of embryos from either cotyledons or young first leaves. Cotyledonand first-leaf-derived calluses pro...
متن کاملSomatic Embryogenesis and Plant Regeneration from Embryonic Axes and Cotyledons Explants of Tea (Camellia sinenesis L.)
In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0...
متن کاملSomatic Embryogenesis and Plant Regeneration in Evening Primrose (Oenothera biennis L.)
Callus induction responses and regeneration through callus-mediated SE were studied from evening primrose (Oenothera biennis L.) as an important medicinal plant of Onagraceae mainly known for its gamma-linoleic acid (GLA) content. The effects of cytokinins, 6-Benzylaminopurine (BAP) and N-(2-furfurylamino)1-H-purine-6-amine [Kinetin (Kin)] and their concentrations (0, 0.5, 1.0 and 1.5 mg/l) in ...
متن کاملSomatic embryogenesis and plant regeneration in Gloriosa L.
Friable callus was initiated from shoot apices of Gloriosa superba L. on basal MS medium supplemented with 2, 4-D (4mg L(-1)) + Kn(5 mg L(-1)) + CH(10 mg L(-1)) + CW(20%). Subculture of callus on the same medium after 4-5 weeks showed induction of large number of somatic embryos, which was confirmed with histological studies. Development of embryoids in plantlet took place when the embryogenic ...
متن کاملSomatic Embryogenesis and Efficient Plant Regeneration in Japanese Cypresses
There are six species in the genus Chamaecyparis worldwide, of which two, namely the Hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.) and Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.) are distributed in Japan (Maruyama et al., 2002). The Hinoki cypress is one of the most important commercial timber trees in Japan, representing about 25% of the plantation area in the country. Howeve...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of cell and molecular researchجلد ۴، شماره ۲، صفحات ۸۱-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023