downregulation of mmp2 and bcl-2 in adipose derived stem cells (ascs) following transfection with ip-10 gene
نویسندگان
چکیده
background: mesenchymal stem cells (mscs) are recently introduced as novel immunological gene carriers for treatment of cancer. it is believed that balance between the expression of angiogenic and anti-angiogenic factors, such as sdf-1 and ip-10, may regulate neovascularization within the tumor. methods: in this study, we compared the expression of important tumor promoting mediators in ip-10-transfected adipose derived stem cells (ascs) to those transfected with sdf-1. ascs were isolated from adipose tissue of a normal subject undergoing cosmetic mamoplasty surgery using collagenase. ascs were transfected with ip-10 or sdf-1 propagated plasmids by electroporation method and lipofectamin 2000. expressions of sdf-1, cxcr4, ip-10, bcl-2, mmp2, il-10, igf-1, and vegf were detected in transfected ascs using quantitative real-time polymerase chain reaction (qrt-pcr). results: results showed that the expressions of sdf-1, cxcr4, bcl-2, mmp2, il-10, igf-1, and vegf were upregulated in sdf-1-transfected ascs. in contrast, bcl-2 and mmp2 transcripts showed 45×103 and 10 fold lower expression in ascs transfected with ip-10 compared to non-transfected cells. conclusion: anti-angiogenic chemokines such as ip-10 may modulate tumor promoting properties of ascs and would be introduced as novel candidates for tumor immunotherapy; however, further studies are needed to be conducted.
منابع مشابه
Downregulation of MMP2 and Bcl-2 in Adipose Derived Stem Cells (ASCs) following Transfection with IP-10 Gene
BACKGROUND Mesenchymal Stem Cells (MSCs) are recently introduced as novel immunological gene carriers for treatment of cancer. It is believed that balance between the expression of angiogenic and anti-angiogenic factors, such as SDF-1 and IP-10, may regulate neovascularization within the tumor. METHODS In this study, we compared the expression of important tumor promoting mediators in IP-10-t...
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عنوان ژورنال:
avicenna journal of medical biotechnologyجلد ۶، شماره ۱، صفحات ۲۷-۳۷
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