the frequency of human leukocyte antigen-drb1 alleles, using sequence-based genotyping in 68 parents-child trios study in iranian subjects

background: the human leukocyte antigen-drb1 (hla-drb1) locus is one of the most polymorphic human loci and has a crucial role in the immune system. assessing the allelic frequencies of hla-drb1 locus would be a fundamental factor in defining the origin of populations, relationships with other populations, disease association studies and the constitution of unrelated bone marrow donor registries. in the current study hla-drb1 alleles and their frequencies are determined in a family-based study by dna sequencing-based typing high-resolution (2 field) level of typing. materials and methods: genomic dna from 3 members of 68 unrelated families (a total of 204 individuals) was extracted. exon 2 of drb1 gene was amplified and sequenced and allele assignment was performed using assign™ sbt v4.7sequence analysis software.   results: we had drb1*11:04 with frequency of 0.0931, drb1*03:01 with 0.0882, drb1*11:01 with 0.0735, drb1*13:01 with 0.071 and also alleles drb1*08:03, drb1*13:42, drb1*14:04 and drb1*14:07 with frequency of 0.0024.   conclusion: a total of 34 different alleles were found in the study subjects with drb1*11:04, drb1*03:01, drb1*11:01 being the most frequent alleles respectively. k e y w o r ds: genotype, hla-drb1, iran, hla-typing