use of hydro-lysates from thunnus tonggol viscera as a peptone for listeria monocytogenes gowth media

نویسندگان

m. alinejhad

b. shabanpour

r. safari

m. alinejhad

چکیده

the objective of this study was to produce fish peptone from tuna (thunnus tonggol) viscera, by alcalase. response surface methodology (rsm) was employed for optimizing the temperature and ph. hydrolysis was done in different tempratures (50-65˚c) , ph (8-8. 5) and selected 13 treatments. samples with higher value of protein were used instead of the standard peptones which applied in commercial media for listeria monocytogenes. based on the three-dimensional graphs, the optimum condition for temperature and ph were determined to be 50˚c and 8. 5 respectively. the results showed that the highest (76. 89 g/l) and the lowest (38. 54 g/l) rates of protein content were related to ph 8 at 57˚c and ph 8 at 50˚c. maximum bacterial growth rate was related to ph 8 at 65˚c. results also showed that tuna (thunnus tonggol) viscera can be used as low cost nitrogen sources for listeria monocytogenes growth media.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation of Listeria monocytogenes from Meat and Dairy Products

Introduction: This study was intended to determine the presence and distribution of Listeria monocytogenes in various meat and dairy products from Qazvin Province by culture followed by biochemical and morphological assays. The identity of the isolates was further obtained by amplification of prfA gene in bacteria isolates. This gene is a transcriptional activator of virulence gene ex...

متن کامل

Partial acid hydro lysates of proteins; assay of liquid protein hydro lysates in protein-depleted rats.

Experiments to determine the comparative nutritive value of partial acid hydrolysates of various proteins have been carried out for several years in this laboratory. Most of this work has involved studies of nitrogen balance in dogs wherein the hydrolysates were administered intravenously. Short term experiments, i.e. 4 to 5 days, were used to determine minimum nitrogen levels at wb.ich the N b...

متن کامل

Isolation and Evaluation of Collagen from the Fish (Thunnus Tonggol) Skin: A Biological Material for Medical Tissue Engineering

Introduction: Collagen bears many applications in pharmacy and medicine, health and cosmetic products as well as food industry. In recent years, much attention has been paid to separation of collagen from marine organisms arising from the fact that its use in the diet is not restricted and triggers no risk of contagious diseases as well as religious restrictions. Moreover, fish collagen is uniq...

متن کامل

The investigation of molecular characterization of presumptive Listeria monocytogenes isolates from a food-processing environment

Background: Listeria is a Gram-positive, non-spore forming, facultative anaerobic intracellular bacterium. The most important pathogens in mammals include Listeria monocytogenes and Listeria ivanovii. The former generally causes disease and death in both humans and animals while the latter performs sporadically and primarily causes illness in ruminant...

متن کامل

DETERMINATION OF DOMINANT SEROVARS OF LISTERIA MONOCYTOGENES

Serovars of Lisreria l1Iollocyrogelles were determined. Sera of aborted samples (200) were collected from different hospitals in Tehran and were tested serologically by immunofluorescent antibody methods (IF tests). 137 positive sera were identified. Positive sera were tested against 12 serovars of Listeria l1Iollocytogelles separately. Titers of antibody in patients' sera for all serovars ...

متن کامل

Hyaluronic acid production by Streptococcus zooepidemicus in marine by-products media from mussel processing wastewaters and tuna peptone viscera

BACKGROUND Hyaluronic acid is one of the biopolymers most commonly used by the pharmaceutical industry. Thus, there is an increasing number of recent works that deal with the production of microbial hyaluronic acid. Different properties and characteristics of the fermentation process have been extensively optimised; however, new carbon and protein sources obtained from by-products or cheap subs...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
پژوهش های علوم و صنایع غذایی ایران

جلد ۸، شماره ۳، صفحات ۰-۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023