serological diagnosis of amoebiasis by elisa in human

Serological diagnosis of amoebiasis by immunofluorescence.

A positive serological diagnosis of amoebiasis could be made by immunofluorescence in 66 of 78 established cases, taking a serum titre of 16 or higher as diagnostic: at this level there were no false positives among 94 control sera. The test is simple and may be carried out on amoebic smears stored for several months in 2-octanol. The serological activity is largely confined to the IgG immunogl...

Rapid serological diagnosis of enterovirus 71 infection by IgM ELISA.

Yoshio Tano, Hiroyuki Shimizu*, Masashi Shiomi, Takashi Nakano and Tatsuo Miyamura Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan Poliomyelitis Research Institute, Kumegawa-cho 5-34-4, Higashimurayama-shi, Tokyo 189-0003, Osaka City General Hospital, Miyakojima-hondori 1-13-22, Miyakojima-ku, Osaka 534-0021 and Mie Nati...

Serological diagnosis of FMD in sheep in Basra by ELISA test

This study was performed to detect the antibodies against the virus-infectionassociated antigen (VIAA) in previously diseased and healthy sheep in Basra. The test is valuable in epizootiological surveys because only infected animals with foot and mouth disease virus will give positive reaction without detection of the virus serotypes. 241 sheep sera were collected from 13 suspicious infected sh...

Performance of Leishmania PFR1 recombinant antigen in serological diagnosis of asymptomatic canine leishmaniosis by ELISA

BACKGROUND Leishmania infantum is a protozoan parasite transmitted by phlebotomine sand flies that causes life-threatening disease in humans and dogs. The dog is the primary reservoir of the parasite and early diagnosis of canine leishmaniosis is crucial at the clinical and epidemiological level. The currently available serological tests for CanL diagnostic show limitations therefore the aim of...

Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis.

Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombina...

Comparison of ELISA and Agar Gel Diffusion Techniques in Diagnosis of Human Hydatidosis.