generation of rat embryonic germ cells via inhibition of tgfß and mek pathways

Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways

OBJECTIVE Embryonic germ (EG) cells are the results of reprogramming primordial germ cells (PGC) in vitro. Studying these cells can be of benefit in determining the mechanism by which specialized cells acquire pluripotency. Therefore in the current study we have tried to derive rat EG cells with inhibition of transforming growth factor-β (TGFβ) and mitogen-activated protein kinase kinase (MEK) ...

Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

  Objective(s): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.   Materials and Methods: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence p...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

co-culture of mouse embryonic stem cells with sertoli cells promote in vitro generation of germ cells

objective(s): sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. the present study was designed to analyze the effect of sertoli cells in differentiation of mouse embryonic stem cells (mescs) to germ cells.   materials and methods: a fusion construct composed of a stra8 gene promoter and the coding region of enhanced green fluorescence pro...

A vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells

Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis.  Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...

The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells

Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...