comparison of proteome profiling of two sensitive and resistant field iranian isolates of leishmania major to glucantime® by 2- dimensional electrophoresis.
نویسندگان
چکیده
background: in this study, two-dimensional gel electrophoresis (2-de) method was applied to determine and compare the protein spots expressed in the two field isolates of leishmania major and recovered from the patients who were clinically sensitive and resistant to glucantime® treatment. methods: leishmania parasites were isolated from the cutaneous lesions of two cl infected patients in shiraz, south of iran. the species of the two isolates were identified as l. major using nested-pcr. sensitivity (sh-214s) and resistance (sh-120r) of the two isolates to meglumine antimonite were checked by the standard in vitro assays. both sensitive and resistant l. major isolates were harvested in rpmi 1640 medium. protein extractions were performed using tca/acetone method and the protein spots were determined by a two-dimensional gel electrophoresis (2-de). the gels were stained with silver nitrate and analyzed by image master 2d melanie-6 software. results: about 2967 protein spots were detected. overall, 89 protein spots repre-sented considerable changes of expression in the resistant isolate of l. major compared to the sensitive isolate. of these, 60 and 29 protein spots were up-and down regulated, respectively. in addition, 11 protein spots present in the resistant isolate were noticed to be absent in the sensitive isolate. conclusion: a number of proteins showed significant changes of expression in the drug-resistant l. major ; moreover, the roles of these proteins probably enhanced the parasite resistance to the drug and increased parasite survival in the cells.
منابع مشابه
Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania major to Glucantime® by 2- Dimensional Electrophoresis
BACKGROUND In this study, two-dimensional gel electrophoresis (2-DE) method was applied to determine and compare the protein spots expressed in the two field isolates of Leishmania major and recovered from the patients who were clinically sensitive and resistant to Glucantime® treatment. METHODS Leishmania parasites were isolated from the cutaneous lesions of two CL infected patients in Shira...
متن کاملComparison of the Proteome Profiling of Iranian isolates of Leishmania tropica, L. major and L. infantum by Two-Dimensional Electrophoresis (2-DE) and Mass-spectrometry
BACKGROUND The mechanisms of virulence and species differences of Leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. In Iran, L. major and L. tropica are known as principal agents of cutaneous leishmaniasis, while L. infantum causes visceral leishmaniasis. METHODS As a preliminary study, we compared the proteome mapping of the above thre...
متن کاملcomparison of the proteome profiling of iranian isolates of leish¬mania tropica, l. major and l. infantum by two- dimen¬sional electrophoresis (2-de) and mass-spectrometry
background: the mechanisms of virulence and species differences of leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. in iran, l. major and l. tropica are known as principal agents of cutaneous leishmaniasis, while l. infantum causes visceral leishmaniasis. methods: as a preliminary study, we compared the proteome mapping of the above thre...
متن کاملprotein profiling on meglumine antimoniate (glucantime®) sensitive and resistant l. tropica isolates by 2- dimentional gel electrophoresis: a preliminary study
background: glucantime ® is the first- line drug for the treatment of all forms of leishmaniasis. unfortunately, the prevalence of parasites becoming resistant to glucantime ® is increasing in several parts of the world including iran. as protein is the most important target for drugs in response to a variety of signals including drugs so, it seems expression protein patterns in sensitive and r...
متن کاملa comparison of linguistic and pragmatic knowledge: a case of iranian learners of english
در این تحقیق دانش زبانشناسی و کاربردشناسی زبان آموزان ایرانی در سطح بالای متوسط مقایسه شد. 50 دانش آموز با سابقه آموزشی مشابه از شش آموزشگاه زبان مختلف در دو آزمون دانش زبانشناسی و آزمون دانش گفتار شناسی زبان انگلیسی شرکت کردند که سوالات هر دو تست توسط محقق تهیه شده بود. همچنین در این تحقیق کارایی کتابهای آموزشی زبان در فراهم آوردن درون داد کافی برای زبان آموزان ایرانی به عنوان هدف جانبی تحقیق ...
15 صفحه اولthe evaluation and comparison of two esp textbooks available on the iranian market for teaching english to the students of medicine
abstract this study evaluated and compared medical terminology and english for the students of medicine (ii) as two representatives of the textbooks available on the iranian market for teaching english to the students of medicine. this research was performed on the basis of a teacher’s and a number of students’ attitudes and the students’ needs analysis for two reasons: first, to investigate...
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of parasitologyجلد ۱۰، شماره ۱، صفحات ۱۹-۲۹
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023