genotyping of clinical streptococcus agalactiae strains based on molecular serotype of capsular (cps) gene cluster sequences using polymerase chain reaction
نویسندگان
چکیده
conclusions the results of the current study demonstrated that type iii is the predominant type in hamedan, followed by types v, type ii, type ia, ib and iv, respectively. use of the molecular serotyping (ms) method such as pcr assay as an alternative to conventional serotyping (cs) method leads to accurate, sensitive, specific, and fast typing of gbs isolates. results among the 62 gbs isolates examined, all capsular types except for vi, vii and viii were found. types iii and v were the most prevalent types with a sum of 46 isolates (74.2%). type iii was the predominant type with 35 (56.5%) isolates, followed by type v (11 isolates; 17.7%), type ii (seven isolates; 11.3%), type ia (five isolates; 8.1%) and ib and iv with similar prevalence of two isolates (3.2%). methods in this experimental study we used sixty-two gbs clinical strains isolated from vaginal swabs (n = 16), urine cultures (n = 45) and blood culture (n = 1) of patients, who had referred to educational hospitals and private clinic centers during nine months from june 2013 to february 2014 in hamedan, iran, for genotyping using multiplex pcr assay. background group b streptococcus (gbs) is major cause of serious life threatening infections including sepsis, pneumonia and meningitis in neonates as well as in pregnant women. capsular polysaccharide typing is significant and essential for epidemiological studies of gbs. objectives the aim of the current study was to differentiate genotypes of gbs clinical isolates based on the polymerase chain reaction (pcr) to acquire information about distribution of gbs types in hamedan, iran.
منابع مشابه
genotyping of clinical streptococcus agalactiae strains based on molecular serotype of capsular (cps) gene cluster sequences using pcr assay in hamadan during 2013-2014
background: group b streptococcus (gbs) is the major cause of serious life threatening infections in neonates, pregnant women, and other adults with underlying diseases. capsular polysaccharide typing is a significant way for epidemiological studies of gbs, the pathogenesis, and other studies associated with gbs infections including surveillance programs and vaccine development in future. molec...
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We report a novel sequence of the serotype II capsular locus of group B streptococcus that resolves inconsistencies among the results of various groups and the sequence in GenBank. This locus was found in diverse lineages and presents genes consistent with the complete synthesis of the type II polysaccharide.
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Background: Group B Streptococci (GBS) or Streptococcus agalactiae is one of the most common causes of sepsis and meningitis in neonates and of invasive diseases in pregnant women. It can also cause infectious disease among adults with underlying medical conditions like immunocompromised individuals. Polysaccharide capsule is an important virulence factor. Nine GBS serotypes (Ia, Ib, II to VI...
متن کاملdirect identification of streptococcus agalactiae in vaginal colonization in pregnant women using polymerase chain reaction
conclusions performing only culture method led to missed false negative carriers. therefore, it is recommended that both the pcr assay and conventional culture method be routinely performed to detect gbs in pregnant women accurately. pcr diagnosis demonstrated a shorter turnaround time when compared with the time consuming culture method. results the prevalence of gbs in the 203 collected sampl...
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عنوان ژورنال:
archives of clinical infectious diseasesجلد ۱۲، شماره ۱، صفحات ۰-۰
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