growth kinetics and human growth hormone production of a heat- inducible recombinante cherichia coli during batch fermentation

Evaluation of Heat Induction Strategy for Recombinant Human Growth Hormone Expression in Fed-Batch Fermentation

RT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli

Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...

Using L-arabinose for Production of Human Growth Hormone in Escherichia coli, Studying the Processing of gIII::hGH Precursor

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Heat production and growth kinetics of E. coli K12 from flow calorimetric measurements on chemostat cultures.

The heat production of E. coli K12 growing aerobically in glucose limited chemostat cultures is determined in the range of specific growth rates mu (= dilution rates D) from 0.058 h-1 to 0.852 h-1 for two different glucose concentrations Se in the instream of the chemostat, namely Se1 = 0.3182 g.l-1 and Se2 = 0.6364 g.l-1. Heat production Q and biomass production P per unit of culture volume sh...

The Over-Expression of Biologically Active Human Growth Hormone in a T5-Based System in Escherichia coli, Studying Temperature Effect

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...