effect of four different media on periodontal ligament cells viability of dry- stored dog teeth

statement of the problem: the maintenance of viable periodontal ligament cells is the most important issue in the long-term preservation of avulsed teeth. purpose: the aim of this study was to assess aloe vera as a new storage media in maintaining the cell viability of dry-stored teeth in comparison with soy milk, hank`s balanced salt solution (hbss), and milk. materials and method: twenty one extracted dog premolar teeth were dried for 30 minutes and stored in soy milk, hbss, milk, and aloe vera extract (50%) for 45 minutes (n=6 for each). furthermore, positive and two negative control groups (n=6), corresponding to 0 min, 30 min, and 2-hour drying times were also prepared respectively. the number of viable cells was counted following storage using trypan blue exclusion. data were statistically analyzed using the one-way anova and post hoc tukey-hsd test. results: statistical analysis showed no significant differences in cell viability among aloe vera, soymilk, and hbss- stored teeth; however, they were all superior to milk. conclusion: aloe vera extract can be recommended as a suitable storage media for avulsed teeth. keywords ● aloe vera  ● cell viability  ● periodontal ligament  ● soy milk  ● tooth avulsion