expression of pro-inflammatory genes in lesions and neutrophils during leishmania major infection in balb/c mice

نویسندگان

soheyla akhzari dept. of laboratory sciences, school of veterinary sciences, bu-ali sina university, hamadan, iran

hossein rezvan dept. of laboratory sciences, school of veterinary sciences, bu-ali sina university, hamadan, iran

masoud zolhavarieh dept. of laboratory sciences, school of veterinary sciences, bu-ali sina university, hamadan, iran dept. of clinical sciences, faculty of para-veterinary sciences, bu-ali sina university, hamedan, iran

چکیده

background: leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries in the world. characterization of inflammatory responses produced in cutaneous leishmaniasis has not yet been completed. methods: the specific primers were designed for ten pro-inflammatory genes including ccl4, ccl3, tnf-α, il-1α, il-12p35, il-12p40, ccl5, ccr5, il-1β and ifn- γ and their expression were assessed and compared using rt-pcr in the lesion and peripheral blood neutrophils in leishmania infected balb/c mice. results: none of the pro-inflammatory genes was expressed in the healthy tissue and except ifn-γ others were down-regulated by the parasite in the lesion in untreated mice. in mice treated with anti-leishmanial drugs, the expression of the pro - inflammatory genes restarted. the figure of pro-inflammatory gene expression in neutrophils was different was from the lesions in treated and untreated mice. conclusion: leishmania is capable to suppress the expression of pro-inflammatory genes in the lesions but not in neutrophils. the expression of tnf-α in the lesions and down-regulation of il-1β in neutrophils could be accounted as an indication for healing of cutaneous leishmaniasis . the results open a new window on characterization of leishmania lesions and clarifying the role of neutrophils in leishmania infections.

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Expression of Pro-inflammatory Genes in Lesions and Neutrophils during Leishmania major Infection in BALB/c Mice

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faculty of psychology and social sciences group of anthropology master thesis in major of anthropology

چکیده پایان نامه (شامل خلاصه، اهداف، روش های اجرا و نتایج به دست آمده): کار جمع آوری گو یش های محلی در سال های اخیر شتاب امیدوار کننده ای به خود گرفته است. شاید از بارزترین اهداف جمع آوری گویش های مختلف، ثبت و ضبط آن، جلوگیری از نابودی و مهمتر از همه حل مشکلات دستوری زبان رسمی باشد. دقت در فرآیند های زبانی گویش های محلی نوع ارتباط مردم نواحی مختلف با پیرامون نشان را به ما نشان خواهد داد. از س...

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عنوان ژورنال:
iranian journal of parasitology

جلد ۱۱، شماره ۴، صفحات ۵۳۴-۵۴۱

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