expression of pro-inflammatory genes in lesions and neutrophils during leishmania major infection in balb/c mice
نویسندگان
چکیده
background: leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries in the world. characterization of inflammatory responses produced in cutaneous leishmaniasis has not yet been completed. methods: the specific primers were designed for ten pro-inflammatory genes including ccl4, ccl3, tnf-α, il-1α, il-12p35, il-12p40, ccl5, ccr5, il-1β and ifn- γ and their expression were assessed and compared using rt-pcr in the lesion and peripheral blood neutrophils in leishmania infected balb/c mice. results: none of the pro-inflammatory genes was expressed in the healthy tissue and except ifn-γ others were down-regulated by the parasite in the lesion in untreated mice. in mice treated with anti-leishmanial drugs, the expression of the pro - inflammatory genes restarted. the figure of pro-inflammatory gene expression in neutrophils was different was from the lesions in treated and untreated mice. conclusion: leishmania is capable to suppress the expression of pro-inflammatory genes in the lesions but not in neutrophils. the expression of tnf-α in the lesions and down-regulation of il-1β in neutrophils could be accounted as an indication for healing of cutaneous leishmaniasis . the results open a new window on characterization of leishmania lesions and clarifying the role of neutrophils in leishmania infections.
منابع مشابه
Expression of Pro-inflammatory Genes in Lesions and Neutrophils during Leishmania major Infection in BALB/c Mice
BACKGROUND Leishmaniasis is a worldwide disease prevalent in tropical and sub-tropical countries in the world. Characterization of inflammatory responses produced in cutaneous leishmaniasis has not yet been completed. METHODS The specific primers were designed for ten pro-inflammatory genes including CCL4, CCL3, TNF-α, IL-1α, IL-12P35, IL-12P40, CCL5, CCR5, IL-1β and IFN- γ and their expressi...
متن کاملExpression of pro-inflammatory genes in lesions, spleens and blood neutrophils after burn injuries in mice treated with silver sulfodiazine
Objective(s): It is now supposed that cytokines released during the burn injuries have a great impact on the immunological and pathological responses after the burn. The main objective of this study was to investigate the expression of some pro-inflammatory genes in the wound, spleen and blood neutrophils during the healing process of burn wounds in a murine model. Materials and Methods: The ex...
متن کاملComparison of Pro-inflammatory Gene Expression Profile in Spleen and Peripheral Blood Neutrophils of BALB/C Mice Infected with Leishmania Major
Introduction: Leishmaniasis is one of the common diseases between humans and animals. The present study was to investigate the expression pattern of pro-inflammatory genes in different stages of cutaneous leishmaniasis progression in order to provide a standard framework for diagnosis of various stages of the disease. Methods: In this experimental study, 2×106 Leishmania major were injected in...
متن کاملExpression of pro-inflammatory genes in lesions, spleens and blood neutrophils after burn injuries in mice treated with silver sulfodiazine
OBJECTIVES It is now supposed that cytokines released during the burn injuries have a great impact on the immunological and pathological responses after the burn. The main objective of this study was to investigate the expression of some pro-inflammatory genes in the wound, spleen and blood neutrophils during the healing process of burn wounds in a murine model. MATERIALS AND METHODS The expr...
متن کاملfaculty of psychology and social sciences group of anthropology master thesis in major of anthropology
چکیده پایان نامه (شامل خلاصه، اهداف، روش های اجرا و نتایج به دست آمده): کار جمع آوری گو یش های محلی در سال های اخیر شتاب امیدوار کننده ای به خود گرفته است. شاید از بارزترین اهداف جمع آوری گویش های مختلف، ثبت و ضبط آن، جلوگیری از نابودی و مهمتر از همه حل مشکلات دستوری زبان رسمی باشد. دقت در فرآیند های زبانی گویش های محلی نوع ارتباط مردم نواحی مختلف با پیرامون نشان را به ما نشان خواهد داد. از س...
Route of Infection Affects Pathogenicity of Leishmania major in BALB/c Mice
Introduction: The term Leishmaniasis applies to a group of parasitic diseases caused by the genus Leishmania. Route of infection is one of the variables that have been reported to influence the immune responses as well as the disease outcome in experimental models of leishmaniasis. This research aims to study the effect of route of infection on the pathogenicity of Leishmania major in BALB/c mi...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۱۱، شماره ۴، صفحات ۵۳۴-۵۴۱
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