association of the bola-drb3 alleles with peripheral blood mononuclear cell proliferation in response to staphylococcusaureus

the aim of this study was to investigate the association of bovine leukocyte antigen-drb3 alleles with peripheral blood mononuclear cell (pbmc) proliferation in response to staphylococcus aureus. the animals included in this study (n=347) were approximately of same age and comprised of f2 holstein-friesian ´ charolais (n = 155), holstein-friesian backcross (f0 holstein-friesian dams crossed with unrelated f1 sires, n = 60), charolais backcross (f1 dams crossed with f0 charolais sire, n=46) and pure holstein-friesian (n = 86).  a sequence-based typing method was used in order to determine the genotype of the animals at bola-drb3 locus and a linear mixed model was used to evaluate the association of bovine leukocyte antigen-drb3 alleles with peripheral blood mononuclear cell (pbmc) proliferation. beside the bola-drb3 alleles, fixed effects of genetic group and gender and random effect of sires were included into the statistical model. in this research, 27 alleles were found for bola-drb3 gene. the results showed that alleles bola-drb3*0101 and bola-drb3*0902 significantly affected on the stimulation index of s. aureus–induced peripheral blood mononuclear cell proliferation (p