production of edible cottonseed protein concentrates by mixed solvent (water: acetone: hexane)

cottonseed is considered among most important crops. it contains about 26 percent oil that ‎in used for vegetable oil production. after oil extraction, the remaining part of the seed know as ‎cottonseed cake with 45 percent protein is routinely used as a protein supplement in feed ‎rations for livestock and less for food use due to presence of gossypol, a toxic constituent of ‎cottonseed.‎ in this study, the production of edible protein concentrate from varamin variety of ‎cottonseed cake was evaluated according to protein advisory group standard by using mixture ‎of water: acetone: hexane in a ratio of (1: 39: 60), (3: 53: 44) and (3: 67: 30) respectively as a ‎solvent as wet extraction‏ ‏of protein. the fiber content, protein content, nitrogen solubility ‎value, oils and free as well as total gossypol contents of all samples were determined. the results ‎showed that the protein concentrate extracted by the mixture of water: acetone: hexane in a ‎ratio of 1: 39: 60 respectively meet the world standards as edible protein.‎