developing michigan cancer foundation 7 cells with stable expression of e7 gene of human papillomavirus type 16
نویسندگان
چکیده
background: human papillomavirus (hpv) is responsible for the development of cervical neoplasia. infection with human papillomavirus type 16 (hpv-16) is a major risk factor for the development of cervical cancer. the virus encodes three oncoproteins (e5, e6 and e7), of which, the e7 oncoprotein is the major protein involved in cell immortalization and transformation of the infected cells. the aim of the current study was to develop michigan cancer foundation 7 (mcf7) cells, which could stably express e7 protein of hpv type 16. methods: e7 gene of hpv type 16 was introduced into mcf7 cells by lipofectamine 2000 reagent and the transfected cells were treated with g418 antibiotic. after antibiotic selection of the transfected cells, stable expression of e7 gene of hpv16 was confirmed by reverse transcriptase polymerase chain reaction (rt-pcr). results: antibiotic selections of transfected cells were performed and transfected cells were alive in cytotoxic concentration of the antibiotic. rna was extracted from transfected cells and e7 gene of hpv16 was amplified by rt-pcr method and a 350-bp band corresponds to e7 was observed. conclusion: results confirmed the stable transfection of cells. the stably transfected cells can be used as a useful tool in future studies on hpv16 and cancers caused by this virus.
منابع مشابه
Developing Michigan Cancer Foundation 7 Cells with Stable Expression of E7 Gene of Human Papillomavirus Type 16
Background: Human papillomavirus (HPV) is responsible for the development of cervical neoplasia. Infection with human papillomavirus type 16 (HPV-16) is a major risk factor for the development of cervical cancer. The virus encodes three oncoproteins (E5, E6 and E7), of which, the E7 oncoprotein is the major protein involved in cell immortalization and transformation o...
متن کاملDeveloping Michigan Cancer Foundation 7 Cells with Stable Expression of E7 Gene of Human Papillomavirus Type 16
BACKGROUND Human papillomavirus (HPV) is responsible for the development of cervical neoplasia. Infection with human papillomavirus type 16 (HPV-16) is a major risk factor for the development of cervical cancer. The virus encodes three oncoproteins (E5, E6 and E7), of which, the E7 oncoprotein is the major protein involved in cell immortalization and transformation of the infected cells. The ai...
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Background: Cervical cancer is the most prevalent tumor in developing countries and the second most frequent cancer among female population worldwide. Specific human papillomaviruses and, most notably, HPV types 16 and 18 are recognized as being caus-ally associated with cervical carcinomas. The early HPV type 16 genes, E6 and E7, di-rectly participate in the in vitro transformation of primary ...
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The contribution of the E6 and E7 open reading frames of human papillomavirus type 6b (HPV6b) and HPV16 to immortalization of human keratinocytes was evaluated by using amphotropic recombinant retroviruses. The HPV16 E7 gene could immortalize primary human keratinocytes without the cooperation of the viral E6 gene; however, E6 was able to contribute significantly to the efficiency of the E7 imm...
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The human papillomavirus type 16 (HPV-16) is a DNA tumor virus highly associated with cervical carcinoma. Viral DNA from HPV-16 is found in primary tumors and their metastatic lesions. To investigate the role of HPV-16 oncoproteins in the development of cancer metastasis, the E6 and E7 genes from HPV-16 were inserted into retrovirus and introduced into nonmetastatic mouse cell lines. Expression...
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عنوان ژورنال:
iranian journal of pathologyناشر: iranian society of pathology
ISSN 1735-5303
دوره 11
شماره 1 2016
میزبانی شده توسط پلتفرم ابری doprax.com
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