a study of microsporidiosis in corneal scrapings of keratitis patients referring to farabi eye hospital, tehran, iran in 2013-14
نویسندگان
چکیده
background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran in 2013-14. materials and methods: two scraping samples were collected from 91 keratitis patients, five cases had prior history of receiving immune suppressive drugs. one of the two collected samples from each participant was used for vero cell culture and the other was used for the preparation of giemsa and gram staining slides. after 30 days, the cells were scrapped and used for dna extraction afterwards, nested polymerase chain reaction (pcr) detection method was applied. primer pairs of small-subunit ribosomal rna gene were designed by clc genomics workbench software to amplify all major microsporidian pathogens, as well as e. bieneusi , which was used as the positive control in this study. results: the nested pcr showed negative results regarding the presence of microsporidia in the samples. similarly, giemsa and gram staining slides did not detect any spores. conclusion: the prevalence of human microsporidiosis ranges between 0% and 50%, worldwide. based on all the negative samples in the present study, we can conclude that the prevalence of this infection among iranian patients falls in the lower quartile. by gathering further evidence, researchers can take a step forward in this area and open new doors for the assessment of aids patients and users of immunosuppressive drugs.
منابع مشابه
A study of microsporidiosis in corneal scrapings of keratitis patients referring to Farabi Eye Hospital, Tehran, Iran in 2013-14
Background and Purpose: Microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. The prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. In the present study, we aimed to investigate microsporidial keratitis in patients referring to Farabi Eye Hospital Tehran, Iran ...
متن کاملA study of microsporidiosis in corneal scrapings of keratitis patients referring to Farabi Eye Hospital, Tehran, Iran in 2013-14
BACKGROUND AND PURPOSE Microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. The prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. In the present study, we aimed to investigate microsporidial keratitis in patients referring to Farabi Eye Hospital Tehran, Iran i...
متن کاملfrom linguistics to literature: a linguistic approach to the study of linguistic deviations in the turkish divan of shahriar
chapter i provides an overview of structural linguistics and touches upon the saussurean dichotomies with the final goal of exploring their relevance to the stylistic studies of literature. to provide evidence for the singificance of the study, chapter ii deals with the controversial issue of linguistics and literature, and presents opposing views which, at the same time, have been central to t...
15 صفحه اولAssessment of High Reliability Organizations Model in Farabi Eye Hospital, Tehran, Iran
Background A high-reliability organization (HRO) is a separate paradigm can indicate medical error reduction and patient safety improvement. Hospitals, as vital organizations in the health care system, can transform to HROs to achieve optimal performance and maximum safety in order to manage unpredicted events efficiently. Therefore, the aim of this research was to determine the knowledge of ma...
متن کاملa study of baudrillards ideas in brian moores fiction
پیدایش مرحله ی جدیدی از نظام سرمایه داری بعد از جنگ جهانی دوم همزمان است با ظهور عصر اطلاعات و رسانه جمعی. در چنین جامعه ای سیر آزادانه ی نشانه ها در فضای بی اساس مجازی بر سرعت فرسایش واقعیت می افزاید. به اعتقاد بودریار، فقدان واقعیت به واسطه ی شبیه سازی آن و تولید حاد واقعیت (hyperreality) پنهان خواهد ماند. این پژوهش بر آن است که جامعه فرانوین توصیف شده در سه رمان بریان مور را با توجه به نظر...
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
current medical mycologyجلد ۱، شماره ۳، صفحات ۳۹-۴۴
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023