optimum dose of gamma irradiation to inactivate vibrio paraheamolyticus in fresh and freeze-dried
نویسندگان
چکیده
background: vibrio paraheamoloyticus is one the causative agents of vibriosis with high mortality in farmed fish and shrimp and under predisposing conditions. objectives: this study was aimed to assess the effect of gamma irradiation on the inactivation of v. paraheamoloyticus under fresh and freeze-dried conditions. methods: vials of 0.5 ml fresh culture of the bacterial suspensions at 1.5*1010 cfu/ml were subjected to 1, 1.5,2, 2.5, 3 and 4 kgy. also, vials of 0.5 ml freeze-dried culture of the bacterial suspensions at 1.5 x 1010 cfu/ml were subjected to 2, 4, 7 and 8 kgy. the bacterial growth behavior was then evaluated on fresh medium. results: the obtained results showed that the minimum doses of 4 and 10 kgy were sufficient for the inactivation of fresh and freeze-dried bacteria, respectively. conclusions: the result of this study shows that inactivation of v. paraheamolyicus in fresh culture condition requires below half- dosage of gamma ray required for the inactivation of the freeze- dried of bacterial cells.
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عنوان ژورنال:
تحقیقات دامپزشکیجلد ۷۰، شماره ۲، صفحات ۱۲۵-۱۳۰
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