immunization with kmp11-ntgp96-gfp fusion of leishmania major induced th1 platform immune response in susceptible balb/c mice

conclusions in conclusion, pegfp-kmp-gp96 (fusion) induced th1 platform immune response against leishmania major (mrho/ir/75/er) infection. results according to the results, the level of cytokines interferon-gamma (ifn-γ) and interleukin (il)-4, and ratio of ifn-γ /il4 was changed among different groups of mice before and seven weeks after immunization. the cytokines were increased significantly in the groups that received pegfp-kmp and pegfp-kmp-gp96 (fusion) compared to pbs and pegfp-n1 groups (p < 0.05). objectives in the present study, the cellular immunogenicity of the pegfp-n1, pegfp-kmp and kmp11-ntgp96-gfp fusion in induction of th1 platform immune response was evaluated in susceptible balb/c mice. methods recombinant plasmid construction of pegfp-n1, pegfp-kmp and kmp11-ntgp96-gfp fusion was prepared. the mice were assigned to four groups (each 15) and immunization was performed with three times (0, 21 and 42 days) with pbs, pegfp-n1, pegfp-kmp and pegfp-kmp-gp96 (fusion), subcutaneously via footpad. cytokines assay was performed a day before and seven weeks following immunization. background leishmaniasis has been known as an endemic disease since ancient times in iran. cell-mediated immunity plays a decisive role in leishmaniasis. a promising vaccine against leishmania could stimulate the th1 immune response.