effect of genotype, explant type and 2,4-d on cell dedifferentiation and callus induction in cumin (cuminum cyminum) medicinal plant
نویسندگان
چکیده
context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replications was conducted on ms medium supplemented with different concentrations of 2,4-d (0, 0.5, 1 and 2 mg/l) plus 0.1 mg/l kinetin in different explants (root, shoot, leaf, embryo and seed) of cumin accessions. in this experiment the evaluated traits include days to callus induction (dci), callus induction percentage (cip) and callus growth rate (cgr). results : statistical analysis showed that seed (as the latest) and root (as the earliest) explants require to 54 and 11 days in order to callus induction initiation respectively. results showed that accession, explant, accession × explant and 2,4-d × explant interactions were statistically significant effects ( p<0.01) on callus induction percentage and callus growth rate traits. furthermore, 2,4-d had a high significant effect on callus induction percentage. conclusion : according to results of this study, root explant is an appropriate explant for large production of callus in some accessions of cuminum cyminum . also, koohbanan accession produced callus in minimum time and afghanistan accession produced high percentage of callus.
منابع مشابه
Effect of Genotype, Explant Type and 2,4-D on Cell Dedifferentiation and Callus Induction in Cumin (Cuminum cyminum L.) Medicinal Plant
Introduction Cumin (Cuminum cyminum L.) is a commercial plant which is valued for its aroma and its medicinal and therapeutic properties. Although India has been the primary exporter of cumin seeds and cumin oil in the world. However Turkey and Iran are providing stiff competition now. India, Turkey, Syria, China, the US, Iran, Indonesia, Sudan, Egypt, Morocco, Algeria, and Libya are the leadin...
متن کاملEffects of Plant Growth Regulators and Explant on Callus Induction in Cuminum cymium L.
Cumin (Cuminum cyminum L.) as a member of the Apiaceae family is one of the most important medicinal plants in Iran. The purpose of this study is to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. For this purpose the cumin seeds (Kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on MS basal medium. Leaf and hypo...
متن کاملeffects of plant growth regulators and explant on callus induction in cuminum cymium l.
cumin (cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in iran. the purpose of this study was to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. for this purpose the cumin seeds (kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on ms basal medium. leaf and hyp...
متن کاملEffects of explant type and growth regulators on callus induction in four ecotypes of Persian shallot
Allium hirtifolium commonly known as Persian shallot is an important wild medicinal plant distributed from North West to central and South West of Iran. To establish an efficient protocol for callus induction, the effects of explant type and growth regulators on callus induction in four ecotypes of Persian shallot were evaluated. Two explants types included basal plates and young leave...
متن کاملEffect of Genotype, Explant Size and Position on Callus Induction in Cereus Peruvianus Mill. (cactaceae)
Cereus peruvianus (Cactaceae) is an important medical plant. The study was carried out on callus induction of Cereus jamacaru f. monstrosus and Cereus hildmannianus fma monstrosa (Cereus peruvianus). Apical and lateral explants were cultured on Murashige and Skoog media with factorial combinations of the auxins indole-3acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and N-(2-furanyl-...
متن کاملAn artificial intelligence approach for modeling volume and fresh weight of callus - A case study of cumin (Cuminum cyminum L.).
Cumin (Cuminum cyminum Linn.) is valued for its aroma and its medicinal and therapeutic properties. A supervised feedforward artificial neural network (ANN) trained with back propagation algorithms, was applied to predict fresh weight and volume of Cuminum cyminum L. calli. Pearson correlation coefficient was used to evaluate input/output dependency of the eleven input parameters. Area, feret d...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
applied biotechnology reportsجلد ۲، شماره ۳، صفحات ۲۶۵-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023