antibacterial activity of salvia reuterana constituents

two flavonids: 5-hydroxy-6,7,4 ́-trimethoxy flavone (salvigenin), 5-hydroxy-7,4 ́-dimethoxy flavone(thitonine) and a labdane type diterpenoid: 8,13-epoxy-14 labdene (manoyl oxide) isolated from the solventextract of the aerial part of salvia reuterana boiss.the proposed structure were stablished by 1hnmr,13cnmr, 2dnmr( c-h-cosy, hmqc),dept and mass spectroscopy and confirmed in accordance withbibiliographic data and computational chemistry results. the three components were evaluated against 7microorganisms including gram(+/-)bacterias by measurment of growth inhibitory zone. they showedsignificant effect specially on gram(-) bacterias and in this way the most effective component was thitoninewhich was even more effective than gentamicine as standard antibiotic.