genotypic and allelic frequencies of igf1 and igf2 genes in broilers analysed by using pcr‐rflp techniques

a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) test was performed to investigate the allele frequencies of the insulin-like growth factor (igf1 and igf2 genes) in 90 broilerchickens. a 793 bp fragment of igf1 gene and 1146 bp fragment of igf2 gene were amplified and digested with hinfi and hsp92ii restriction enzymes,respectively. two types of alleles of a and b and three types of genotypes of aa, ab and bb were observed in both genes. the results indicated predominant b-allele (0.85) was higher for igf1 buta-allele (0.5611) igf2 in the broiler population.