mapping of fertility restorer genes (rf) using microsatellite markers (ssr) in rice
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منابع مشابه
validation of ssr markers linked to restoring fertility (rf) genes and genotyping of rice lines at rf loci
the present study was carried out with the objective of validating linked ssr markers to rf genes and adopting marker assisted selection (mas) for restorer/non-restorer line detection in wild abortive (wa) type of cytoplasmic male sterility (cms). twelve ssr markers reported to be linked to rf genes were analyzed in the mapping population of nedaa/pajouhesh. among these, three markers, namely, ...
متن کاملInheritance of the fertility restoration and genotyping of rice lines at the restoring fertility (Rf) loci using molecular markers
The combination of cytoplasmic male sterility (CMS) in one parent and a restorer gene (Rf) to restore fertility in another are indispensable for the development of hybrid varieties. To genotype rice lines at the restoring fertility (Rf) loci, 38 lines were crossed with a sterile tester (rfrf) line. Pollen fertility test was performed to identify sterile and fertile F1 hybrids. Seven lines were ...
متن کاملMapping fertility-restoring genes of rice WA cytoplasmic male sterility using SSLP markers
Rice wild abortive (WA) type cytoplasmic male sterility (CMS) is commercially used for production of hybrid seeds in China. Two fertility restorer genes in the CMS-WA system were detected with SSLP markers in this study. An F 2 population consisting of 210 excessive sterile individuals from a cross between Zhenshan 97A and a strong restorer line IR24 was used for mapping of Rf4. The genetic dis...
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Salinity stress is one of the most widespread soil problems next to drought, in rice growing areas. ReducingSodium (Na+), while maintaining Potassium (K+) uptake in rice are traits that would aid in salinity tolerance.Therefore, the identification of quantitative trait loci (QTLs) associated with those for Na+ and K+uptake, will enable breeders to use marker-assisted selection...
متن کاملGenetic variation of Garra rufa fish in Kermanshah and Bushehr provinces, Iran, using SSR microsatellite markers
Six highly variable microsatellite loci were used to investigate the genetic diversity and population structure of the Garra rufa in Kermanshah and Bushehr provinces, Iran. All of the 6 microsatellite loci screened in this study showed polymorphism. A total of 90 individual fish from 3 populations were genotyped and 60 alleles were observed in all loci. The number of alleles per locus ranged fr...
متن کاملEvolutionary plasticity of restorer-of-fertility-like proteins in rice
Hybrid seed production in rice relies on cytoplasmic male sterility (CMS) induced by specific mitochondrial proteins, whose deleterious effects are suppressed by nuclear Restorer of Fertility (RF) genes. The majority of RF proteins belong to a specific clade of the RNA-binding pentatricopeptide repeat protein family. We have characterised 'restorer-of-fertility-like' (RFL) sequences from 13 Ory...
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علوم گیاهان زراعی ایرانجلد ۴۰، شماره ۱، صفحات ۰-۰
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