hollow fiber-based liquid phase microextraction combined with high-performance liquid chromatography for extraction and determination of loratadine in human plasma

the applicability of hollow fiber-based liquid phase microextraction (hf-lpme) was evaluated for the extraction and preconcentration of loratadine prior to determination by hplc. in order to obtain high extraction recovery, the parameters affecting the hf-lpme including type of organic phase, ph of the source and receiving phases, stirring rate, extraction time and salt addition were studied and optimized. under the optimized conditions, enrichment factor of 308 with relative standard deviation (r.s.d.%) equel 3.8% were achieved. the calibration curve was linear in the range of 0.3–600 ng/ml with good linearity (r2 = 0.9991). the limit of detection (lod) was 0.1 ng/ml (based on s/n = 3). finally, determination of loratadine in human plasma was used by hf-lpme-hplc and indicated that this method had an excellent clean-up and high recovery factor (84%) for loratadine.