a comparison of exogenous application of benzyladenine (ba) and chlormequate (ccc) on embryo germination in stenospermic grape through embryo rescue method
نویسندگان
چکیده
consumers prefer seedless grapes for table and raisin grape industry. release of new superior seedless grape cultivars is one of the main objectives in grape breeding programs. embryo rescue is one of the techniques to develop a new seedless cultivar. therefore, in this study, the effects of bud flower spraying with ba (50 ppm) and ccc (200 ppm) before blooming were studied in ‘flame seedless’ ‘perlette’ and ‘askari’. ovules were extracted 50 days past pollination, cultured on medium and then their embryos were dissected out of ovules and cultured in nn medium. recovery and germination of embryos were evaluated. spraying flowers with ba and ccc resulted in 32.94 and 32.05% of recovered embryos and 26.76 and 27.05% of germinated embryos, respectively, which both had significant differences with control.
منابع مشابه
Improved in vitro embryo development of stenospermic grape by putrescine.
The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed's culture medium. In Perlón x Argentina, 2 mM of putrescine statistically incre...
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15 صفحه اولApplication of Embryo Rescue Technique in Juglans regia L. x J. nigra L. Hybridization
Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...
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عنوان ژورنال:
علوم باغبانی ایرانجلد ۴۳، شماره ۳، صفحات ۲۶۷-۲۷۴
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